View source for 4bag


==Feruloyl Esterase Domain of XYNY from Clostridium thermocellum after exposure to 266nm UV laser==
<StructureSection load='4bag' size='340' side='right'caption='[[4bag]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bag OCA], [https://pdbe.org/4bag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bag RCSB], [https://www.ebi.ac.uk/pdbsum/4bag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bag ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XYNY_ACETH XYNY_ACETH] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation- damage induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures.,Pereira PJ, Royant A, Panjikar S, de Sanctis D J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456<ref>PMID:23178456</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bag" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acetivibrio thermocellus]]
[[Category: Large Structures]]
[[Category: Pereira PJB]]
[[Category: De Sanctis D]]