View source for 4bjr


==Crystal structure of the complex between Prokaryotic Ubiquitin-like Protein Pup and its Ligase PafA==
<StructureSection load='4bjr' size='340' side='right'caption='[[4bjr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bjr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BJR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bjr OCA], [https://pdbe.org/4bjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bjr RCSB], [https://www.ebi.ac.uk/pdbsum/4bjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bjr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PUP_CORGL PUP_CORGL] Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation.[HAMAP-Rule:MF_02106]<ref>PMID:22910360</ref> [https://www.uniprot.org/uniprot/PAFA_CORGL PAFA_CORGL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Prokaryotic ubiquitin-like protein (Pup) is covalently attached to target proteins by the ligase PafA, tagging substrates for proteasomal degradation. The crystal structure of Pup in complex with PafA, reported here, reveals that a long groove wrapping around the enzyme serves as a docking site for Pup. Upon binding, the C-terminal region of the intrinsically disordered Pup becomes ordered to form two helices connected by a linker, positioning the C-terminal glutamate in the active site of PafA.

Crystal Structure of the Complex between Prokaryotic Ubiquitin-like Protein and Its Ligase PafA.,Barandun J, Delley CL, Ban N, Weber-Ban E J Am Chem Soc. 2013 Apr 23. PMID:23601177<ref>PMID:23601177</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bjr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Corynebacterium glutamicum]]
[[Category: Large Structures]]
[[Category: Ban N]]
[[Category: Barandun J]]
[[Category: Delley CL]]
[[Category: Weber-Ban E]]