View source for 4bk0


==Crystal structure of the KIX domain of human RECQL5 (domain-swapped dimer)==
<StructureSection load='4bk0' size='340' side='right'caption='[[4bk0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bk0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BK0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bk0 OCA], [https://pdbe.org/4bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bk0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RECQ5_HUMAN RECQ5_HUMAN] May have an important role in DNA metabolism.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positioned to sterically block elongation. The crystal structure of the RECQL5 KIX domain reveals similarities with TFIIS, and binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. Together, our findings reveal a dual mode of transcriptional repression by RECQL5 that includes structural mimicry of the Pol II-TFIIS interaction.

Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.,Kassube SA, Jinek M, Fang J, Tsutakawa S, Nogales E Nat Struct Mol Biol. 2013 Jun 9. doi: 10.1038/nsmb.2596. PMID:23748380<ref>PMID:23748380</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bk0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fang J]]
[[Category: Jinek M]]
[[Category: Kassube SA]]
[[Category: Nogales E]]
[[Category: Tsutakawa S]]