View source for 4bol


==Crystal structure of AmpDh2 from Pseudomonas aeruginosa in complex with pentapeptide==
<StructureSection load='4bol' size='340' side='right'caption='[[4bol]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BOL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=J0J:D-ALANYL-N-[(2S,6R)-6-AMINO-6-CARBOXY-1-{[(1R)-1-CARBOXYETHYL]AMINO}-1-OXOHEXAN-2-YL]-D-GLUTAMINE'>J0J</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bol OCA], [https://pdbe.org/4bol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bol RCSB], [https://www.ebi.ac.uk/pdbsum/4bol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bol ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HT86_PSEAE Q9HT86_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The zinc protease AmpDh2 is a virulence determinant of Pseudomonas aeruginosa , a problematic human pathogen. The mechanism of how the protease manifests virulence is not known, but it is known that it turns over the bacterial cell wall. The reaction of AmpDh2 with the cell wall was investigated, and nine distinct turnover products were characterized by LC/MS/MS. The enzyme turns over both the cross-linked and noncross-linked cell wall. Three high-resolution X-ray structures, the apo enzyme and two complexes with turnover products, were solved. The X-ray structures show how the dimeric protein interacts with the inner leaflet of the bacterial outer membrane and that the two monomers provide a more expansive surface for recognition of the cell wall. This binding surface can accommodate the 3D solution structure of the cross-linked cell wall.

Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa.,Martinez-Caballero S, Lee M, Artola-Recolons C, Carrasco-Lopez C, Hesek D, Spink E, Lastochkin E, Zhang W, Hellman LM, Boggess B, Mobashery S, Hermoso JA J Am Chem Soc. 2013 Jul 8. PMID:23819763<ref>PMID:23819763</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bol" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Artola-Recolons C]]
[[Category: Boggess B]]
[[Category: Carrasco-Lopez C]]
[[Category: Hellman LM]]
[[Category: Hermoso JA]]
[[Category: Hesek D]]
[[Category: Lastochkin E]]
[[Category: Lee M]]
[[Category: Martinez-Caballero S]]
[[Category: Mobashery S]]
[[Category: Spink EE]]
[[Category: Zhang W]]