View source for 4brm


==Sulfur SAD phasing of the Legionella pneumophila NTPDase1 - crystal form III (closed) in complex with sulfate==
<StructureSection load='4brm' size='340' side='right'caption='[[4brm]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4brm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BRM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bqz|4bqz]], [[4br0|4br0]], [[4br2|4br2]], [[4br4|4br4]], [[4br5|4br5]], [[4br7|4br7]], [[4br9|4br9]], [[4bra|4bra]], [[4brc|4brc]], [[4brd|4brd]], [[4bre|4bre]], [[4brf|4brf]], [[4brg|4brg]], [[4brh|4brh]], [[4bri|4bri]], [[4brk|4brk]], [[4brl|4brl]], [[4brn|4brn]], [[4bro|4bro]], [[4brp|4brp]], [[4brq|4brq]], [[3cj1|3cj1]], [[3cj7|3cj7]], [[3cj9|3cj9]], [[3cja|3cja]]</div></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Apyrase Apyrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.5 3.6.1.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4brm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4brm OCA], [https://pdbe.org/4brm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4brm RCSB], [https://www.ebi.ac.uk/pdbsum/4brm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4brm ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In vertebrates, membrane-bound ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) on the cell surface are responsible for signal conversion and termination in purinergic signaling by extracellular nucleotides. Here we present apo and complex structures of the rat NTPDase2 extracellular domain and Legionella pneumophila NTPDase1, including a high-resolution structure with a transition-state analog. Comparison of ATP and ADP binding modes shows how NTPDases engage the same catalytic site for hydrolysis of nucleoside triphosphates and diphosphates. We find that this dual specificity is achieved at the expense of base specificity. Structural and mutational studies indicate that a conserved active-site water is replaced by the phosphate product immediately after phosphoryl transfer. Partial base specificity for purines in LpNTPDase1 is based on a different intersubunit base binding site for pyrimidine bases. A comparison of the bacterial enzyme in six independent crystal forms shows that NTPDases can undergo a domain closure motion of at least 17 degrees .

Crystallographic Snapshots along the Reaction Pathway of Nucleoside Triphosphate Diphosphohydrolases.,Zebisch M, Krauss M, Schafer P, Lauble P, Strater N Structure. 2013 Jul 2. pii: S0969-2126(13)00200-1. doi:, 10.1016/j.str.2013.05.016. PMID:23830739<ref>PMID:23830739</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4brm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ectonucleoside triphosphate diphosphohydrolase|Ectonucleoside triphosphate diphosphohydrolase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Apyrase]]
[[Category: Atcc 33152]]
[[Category: Large Structures]]
[[Category: Lauble, P]]
[[Category: Schaefer, P]]
[[Category: Straeter, N]]
[[Category: Zebisch, M]]
[[Category: Adpase]]
[[Category: Atpase]]
[[Category: Cd39]]
[[Category: Domain rotation]]
[[Category: Hydrolase]]
[[Category: Ntpdase]]
[[Category: Purinergic signalling]]
[[Category: Transition state]]