View source for 4bw9


==PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP==
<StructureSection load='4bw9' size='340' side='right'caption='[[4bw9]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bw9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BW9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bw9 OCA], [https://pdbe.org/4bw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bw9 RCSB], [https://www.ebi.ac.uk/pdbsum/4bw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bw9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.

Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins.,Schneider S, Gattner MJ, Vrabel M, Flugel V, Lopez-Carrillo V, Prill S, Carell T Chembiochem. 2013 Sep 11. doi: 10.1002/cbic.201300435. PMID:24027216<ref>PMID:24027216</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bw9" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanosarcina mazei]]
[[Category: Carell T]]
[[Category: Fluegel V]]
[[Category: Gattner MJ]]
[[Category: Lopez-Carillo V]]
[[Category: Schneider S]]
[[Category: Vrabel M]]