View source for 4bz0


==Structural characterization using Sulfur-SAD of the cytoplasmic domain of Burkholderia pseudomallei PilO2Bp, an actin-like protein component of a Type IVb R64-derivative pilus machinery.==
<StructureSection load='4bz0' size='340' side='right'caption='[[4bz0]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bz0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZ0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bz0 OCA], [https://pdbe.org/4bz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bz0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bz0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q63JW5_BURPS Q63JW5_BURPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2Bp, a protein component of the Tfpb R64 thin pilus variant assembly machinery from the pathogen Burkholderia pseudomallei. PilO2Bp belongs to the PF06864 Pfam family, for which an improved definition is presented based on newly derived Hidden Markov Model (HMM) profiles. The 3D structure of the N-terminal domain of PilO2Bp (N-PilO2Bp), here reported, is the first structural representative of the PF06864 family. N-PilO2Bp presents an actin-like ATPase fold that is shown to be present in BfpC, a different variant assembly protein; the new HMM profiles classify BfpC as a PF06864 member. Our results provide structural insight into the PF06864 family and on the Type IV pili assembly machinery.

Redefining the PF06864 Pfam Family Based on Burkholderia pseudomallei PilO2Bp S-SAD Crystal Structure.,Lassaux P, Conchillo-Sole O, Manjasetty BA, Yero D, Perletti L, Belrhali H, Daura X, Gourlay LJ, Bolognesi M PLoS One. 2014 Apr 11;9(4):e94981. doi: 10.1371/journal.pone.0094981. eCollection, 2014. PMID:24728008<ref>PMID:24728008</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bz0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Burkholderia pseudomallei K96243]]
[[Category: Large Structures]]
[[Category: Belrhali H]]
[[Category: Bolognesi M]]
[[Category: Conchillo-Sole O]]
[[Category: Daura X]]
[[Category: Gourlay L]]
[[Category: Lassaux P]]
[[Category: Manjasetty BA]]
[[Category: Perletti L]]
[[Category: Yero D]]