View source for 4ca0


==Structural Basis for the microtubule binding of the human kinetochore Ska complex==
<StructureSection load='4ca0' size='340' side='right'caption='[[4ca0]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ca0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CA0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.259&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ca0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ca0 OCA], [https://pdbe.org/4ca0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ca0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ca0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ca0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SKA1_HUMAN SKA1_HUMAN] Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.<ref>PMID:17093495</ref> <ref>PMID:19289083</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ability of kinetochores (KTs) to maintain stable attachments to dynamic microtubule structures ('straight' during microtubule polymerization and 'curved' during microtubule depolymerization) is an essential requirement for accurate chromosome segregation. Here we show that the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. This contrasts with the Ndc80 complex, which binds straight microtubules by recognizing the dimeric interface of tubulin. The Ska1 microtubule-binding domain interacts with tubulins using multiple contact sites that allow the Ska complex to bind microtubules in multiple modes. Disrupting either the flexibility or the tubulin contact sites of the Ska1 microtubule-binding domain perturbs normal mitotic progression, explaining the critical role of the Ska complex in maintaining a firm grip on dynamic microtubules.

Structural basis for microtubule recognition by the human kinetochore Ska complex.,Abad MA, Medina B, Santamaria A, Zou J, Plasberg-Hill C, Madhumalar A, Jayachandran U, Redli PM, Rappsilber J, Nigg EA, Jeyaprakash AA Nat Commun. 2014 Jan 13;5:2964. doi: 10.1038/ncomms3964. PMID:24413531<ref>PMID:24413531</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ca0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Abad M]]
[[Category: Jayachandran U]]
[[Category: Jeyaprakash AA]]
[[Category: Madhumalar A]]
[[Category: Medina B]]
[[Category: Nigg EA]]
[[Category: Plasberg-Hill C]]
[[Category: Rappsilber J]]
[[Category: Redli PM]]
[[Category: Santamaria A]]
[[Category: Zou J]]