View source for 4cau


==THREE-DIMENSIONAL STRUCTURE OF DENGUE VIRUS SEROTYPE 1 COMPLEXED WITH 2 HMAB 14C10 FAB==
<SX load='4cau' size='340' side='right' viewer='molstar' caption='[[4cau]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cau]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_1 Dengue virus 1] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CAU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cau OCA], [https://pdbe.org/4cau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cau RCSB], [https://www.ebi.ac.uk/pdbsum/4cau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cau ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/Q689G3_9FLAV Q689G3_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS013754_004_099774]
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== Publication Abstract from PubMed ==
Dengue virus (DENV) is a mosquito-borne flavivirus that affects 2.5 billion people worldwide. There are four dengue serotypes (DENV1 to DENV4), and infection with one elicits lifelong immunity to that serotype but offers only transient protection against the other serotypes. Identification of the protective determinants of the human antibody response to DENV is a vital requirement for the design and evaluation of future preventative therapies and treatments. Here, we describe the isolation of a neutralizing antibody from a DENV1-infected patient. The human antibody 14c10 (HM14c10) binds specifically to DENV1. HM14c10 neutralizes the virus principally by blocking virus attachment; at higher concentrations, a post-attachment step can also be inhibited. In vivo studies show that the HM14c10 antibody has antiviral activity at picomolar concentrations. A 7 A resolution cryoelectron microscopy map of Fab fragments of HM14c10 in a complex with DENV1 shows targeting of a discontinuous epitope that spans the adjacent surface of envelope protein dimers. As found previously, a human antibody specific for the related West Nile virus binds to a similar quaternary structure, suggesting that this could be an immunodominant epitope. These findings provide a structural and molecular context for durable, serotype-specific immunity to DENV infection.

The structural basis for serotype-specific neutralization of dengue virus by a human antibody.,Teoh EP, Kukkaro P, Teo EW, Lim AP, Tan TT, Yip A, Schul W, Aung M, Kostyuchenko VA, Leo YS, Chan SH, Smith KG, Chan AH, Zou G, Ooi EE, Kemeny DM, Tan GK, Ng JK, Ng ML, Alonso S, Fisher D, Shi PY, Hanson BJ, Lok SM, Macary PA Sci Transl Med. 2012 Jun 20;4(139):139ra83. PMID:22723463<ref>PMID:22723463</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</SX>
[[Category: Dengue virus 1]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Alonso S]]
[[Category: Aung M]]
[[Category: Chan AH]]
[[Category: Chan SH]]
[[Category: Fisher D]]
[[Category: Hanson BJ]]
[[Category: Kemeny DM]]
[[Category: Kostyuchenko VA]]
[[Category: Kukkaro P]]
[[Category: Leo YS]]
[[Category: Lim AP]]
[[Category: Lok SM]]
[[Category: Macary PA]]
[[Category: Ng JK]]
[[Category: Ng ML]]
[[Category: Ooi EE]]
[[Category: Schul W]]
[[Category: Shi PY]]
[[Category: Smith KG]]
[[Category: Tan GK]]
[[Category: Tan TT]]
[[Category: Teo EW]]
[[Category: Teoh EP]]
[[Category: Yip A]]
[[Category: Zou G]]