View source for 4cfo


==Structure of Lytic Transglycosylase MltC from Escherichia coli in complex with tetrasaccharide at 2.9 A resolution.==
<StructureSection load='4cfo' size='340' side='right'caption='[[4cfo]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cfo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CFO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NM6:(2R)-2-[(2R,3S,4R,5R,6R)-5-ACETAMIDO-2-(HYDROXYMETHYL)-3,6-BIS(OXIDANYL)OXAN-4-YL]OXYPROPANAMIDE'>NM6</scene>, <scene name='pdbligand=NM9:(2R)-2-[(2R,3S,4R,5R,6R)-5-ACETAMIDO-2-(HYDROXYMETHYL)-6-METHOXY-3-OXIDANYL-OXAN-4-YL]OXYPROPANAMIDE'>NM9</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfo OCA], [https://pdbe.org/4cfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cfo RCSB], [https://www.ebi.ac.uk/pdbsum/4cfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MLTC_ECOLI MLTC_ECOLI] Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division.[HAMAP-Rule:MF_01616]
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== Publication Abstract from PubMed ==
The lytic transglycosylases are essential bacterial enzymes that catalyze the nonhydrolytic cleavage of the glycan strands of the bacterial cell wall. We describe here the structural and catalytic properties of MltC, one of the seven lytic transglycosylases found in the genome of the Gram-negative bacterium Escherichia coli. The 2.3 A resolution X-ray structure of a soluble construct of MltC shows a unique, compared to known lytic transglycosylase structures, two-domain structure characterized by an expansive active site of 53 A length extending through an interface between the domains. The structures of three complexes of MltC with cell wall analogues suggest the positioning of the peptidoglycan in the active site both as a substrate and as a product. One complex is suggested to correspond to an intermediate in the course of sequential and exolytic cleavage of the peptidoglycan. Moreover, MltC partitioned its reactive oxocarbenium-like intermediate between trapping by the C6-hydroxyl of the muramyl moiety (lytic transglycosylase activity, the major path) and by water (muramidase activity). Genomic analysis identifies the presence of an MltC homologue in no less than 791 bacterial genomes. While the role of MltC in cell wall assembly and maturation remains uncertain, we propose a functional role for this enzyme as befits the uniqueness of its two-domain structure.

Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli.,Artola-Recolons C, Lee M, Bernardo-Garcia N, Blazquez B, Hesek D, Bartual SG, Mahasenan KV, Lastochkin E, Pi H, Boggess B, Meindl K, Uson I, Fisher JF, Mobashery S, Hermoso JA ACS Chem Biol. 2014 Jul 10. PMID:24988330<ref>PMID:24988330</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4cfo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Artola-Recolons C]]
[[Category: Bernardo-Garcia N]]
[[Category: Hermoso JA]]
[[Category: Mobashery S]]