View source for 4cii


==Crystal structure of N-terminally truncated Helicobacter pylori T4SS Protein CagL as domain swapped dimer==
<StructureSection load='4cii' size='340' side='right'caption='[[4cii]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cii]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CII FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cii OCA], [https://pdbe.org/4cii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cii RCSB], [https://www.ebi.ac.uk/pdbsum/4cii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cii ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O25272_HELPY O25272_HELPY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A new crystal form of the Helicobacter pylori type IV secretion system (T4SS) pilus protein CagL is described here. In contrast to two previously reported monomeric structures, CagL forms a three-dimensional domain-swapped dimer. CagL dimers can arise during refolding from inclusion bodies or can form spontaneously from purified monomeric CagL in the crystallization conditions. Monomeric CagL forms a three-helix bundle, with which the N-terminal helix is only loosely associated. In the new crystal form, the N-terminal helix is missing. The domain swap is owing to exchange of the C-terminal helix between the two protomers of a dimer. A loop-to-helix transition results in a long helix of 108 amino acids comprising the penultimate and the last helix of the monomer. The RGD motif of dimeric CagL adopts an alpha-helical conformation. In contrast to the previously reported structures, the conserved and functionally important C-terminal hexapeptide is resolved. It extends beyond the three-helix bundle as an exposed helical appendage. This new crystal form contributes to the molecular understanding of CagL by highlighting rigid and flexible regions in the protein and by providing the first view of the C-terminus. Based on the structural features, a previously unrecognized homology between CagL and CagI is discussed.

Structure of a three-dimensional domain-swapped dimer of the Helicobacter pylori type IV secretion system pilus protein CagL.,Barden S, Schomburg B, Conradi J, Backert S, Sewald N, Niemann HH Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1391-400. doi:, 10.1107/S1399004714003150. Epub 2014 Apr 30. PMID:24816107<ref>PMID:24816107</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4cii" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Helicobacter pylori 26695]]
[[Category: Large Structures]]
[[Category: Barden S]]
[[Category: Niemann HH]]
[[Category: Schomburg B]]