View source for 4ckq


==X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum==
<StructureSection load='4ckq' size='340' side='right'caption='[[4ckq]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ckq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CKQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ckq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckq OCA], [https://pdbe.org/4ckq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ckq RCSB], [https://www.ebi.ac.uk/pdbsum/4ckq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ckq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A3DJS9_ACET2 A3DJS9_ACET2] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glucuronoxylan endo-beta-1,4-xylanases cleave the xylan chain specifically at sites containing 4-O-methylglucuronic acid substitutions. These enzymes have recently received considerable attention owing to their importance in the cooperative hydrolysis of heteropolysaccharides. However, little is known about the hydrolysis of glucuronoxylans in extreme environments. Here, the structure of a thermostable family 30 glucuronoxylan endo-beta-1,4-xylanase (CtXyn30A) from Clostridium thermocellum is reported. CtXyn30A is part of the cellulosome, a highly elaborate multi-enzyme complex secreted by the bacterium to efficiently deconstruct plant cell-wall carbohydrates. CtXyn30A preferably hydrolyses glucuronoxylans and displays maximum activity at pH 6.0 and 70 degrees C. The structure of CtXyn30A displays a (beta/alpha)8 TIM-barrel core with a side-associated beta-sheet domain. Structural analysis of the CtXyn30A mutant E225A, solved in the presence of xylotetraose, revealed xylotetraose-cleavage oligosaccharides partially occupying subsites -3 to +2. The sugar ring at the +1 subsite is held in place by hydrophobic stacking interactions between Tyr139 and Tyr200 and hydrogen bonds to the OH group of Tyr227. Although family 30 glycoside hydrolases are retaining enzymes, the xylopyranosyl ring at the -1 subsite of CtXyn30A-E225A appears in the alpha-anomeric configuration. A set of residues were found to be strictly conserved in glucuronoxylan endo-beta-1,4-xylanases and constitute the molecular determinants of the restricted specificity displayed by these enzymes. CtXyn30A is the first thermostable glucuronoxylan endo-beta-1,4-xylanase described to date. This work reveals that substrate recognition by both thermophilic and mesophilic glucuronoxylan endo-beta-1,4-xylanases is modulated by a conserved set of residues.

Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.,Freire F, Verma A, Bule P, Alves VD, Fontes CM, Goyal A, Najmudin S Acta Crystallogr D Struct Biol. 2016 Nov 1;72(Pt 11):1162-1173. Epub 2016 Oct 28. PMID:27841749<ref>PMID:27841749</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ckq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acetivibrio thermocellus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Fontes CMGA]]
[[Category: Freire F]]
[[Category: Goyal A]]
[[Category: Najmudin S]]
[[Category: Verma AK]]