View source for 4cml


==Crystal Structure of INPP5B in complex with Phosphatidylinositol 3,4- bisphosphate==
<StructureSection load='4cml' size='340' side='right'caption='[[4cml]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CML FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=52N:1,2-DIOCTANOYL+PHOSPHATIDYL+EPI-INOSITOL+(3,4)-BISPHOSPHATE'>52N</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cml OCA], [https://pdbe.org/4cml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cml RCSB], [https://www.ebi.ac.uk/pdbsum/4cml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cml ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I5P2_HUMAN I5P2_HUMAN] Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.<ref>PMID:7721860</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SHIP2, OCRL, and INPP5B belong to inositol polyphosphate 5-phophatase subfamilies involved in insulin regulation and Lowes syndrome. The structural basis for membrane recognition, substrate specificity, and regulation of inositol polyphosphate 5-phophatases is still poorly understood. We determined the crystal structures of human SHIP2, OCRL, and INPP5B, the latter in complex with phosphoinositide substrate analogs, which revealed a membrane interaction patch likely to assist in sequestering substrates from the lipid bilayer. Residues recognizing the 1-phosphate of the substrates are highly conserved among human family members, suggesting similar substrate binding modes. However, 3- and 4-phosphate recognition varies and determines individual substrate specificity profiles. The high conservation of the environment of the scissile 5-phosphate suggests a common reaction geometry for all members of the human 5-phosphatase family.

Structural Basis for Phosphoinositide Substrate Recognition, Catalysis, and Membrane Interactions in Human Inositol Polyphosphate 5-Phosphatases.,Tresaugues L, Silvander C, Flodin S, Welin M, Nyman T, Graslund S, Hammarstrom M, Berglund H, Nordlund P Structure. 2014 Apr 2. pii: S0969-2126(14)00073-2. doi:, 10.1016/j.str.2014.01.013. PMID:24704254<ref>PMID:24704254</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4cml" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phosphoinositide phosphatase|Phosphoinositide phosphatase]]
*[[3D structures of inositol polyphosphate 5-phosphatase OCRL|3D structures of inositol polyphosphate 5-phosphatase OCRL]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Arrowsmith CH]]
[[Category: Berglund H]]
[[Category: Bountra C]]
[[Category: Edwards AM]]
[[Category: Ekblad T]]
[[Category: Flodin S]]
[[Category: Graslund S]]
[[Category: Karlberg T]]
[[Category: Moche M]]
[[Category: Nordlund P]]
[[Category: Nyman T]]
[[Category: Schuler H]]
[[Category: Silvander C]]
[[Category: Thorsell AG]]
[[Category: Tresaugues L]]
[[Category: Weigelt J]]
[[Category: Welin M]]