View source for 4cu5


==C-terminal domain of endolysin from phage CD27L is a trigger and release factor==
<StructureSection load='4cu5' size='340' side='right'caption='[[4cu5]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cu5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_virus_phiCD27 Clostridium virus phiCD27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CU5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cu5 OCA], [https://pdbe.org/4cu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cu5 RCSB], [https://www.ebi.ac.uk/pdbsum/4cu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cu5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B6SBV8_9CAUD B6SBV8_9CAUD] 
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== Publication Abstract from PubMed ==
The bacteriophage PhiCD27 is capable of lysing Clostridium difficile, a pathogenic bacterium that is a major cause for nosocomial infection. A recombinant CD27L endolysin lyses C. difficile in vitro, and represents a promising alternative as a bactericide. To better understand the lysis mechanism, we have determined the crystal structure of an autoproteolytic fragment of the CD27L endolysin. The structure covers the C-terminal domain of the endolysin, and represents a novel fold that is identified in a number of lysins that target Clostridia bacteria. The structure indicates endolysin cleavage occurs at the stem of the linker connecting the catalytic domain with the C-terminal domain. We also solved the crystal structure of the C-terminal domain of a slow cleaving mutant of the CTP1L endolysin that targets C. tyrobutyricum. Two distinct dimerization modes are observed in the crystal structures for both endolysins, despite a sequence identity of only 22% between the domains. The dimers are validated to be present for the full length protein in solution by right angle light scattering, small angle X-ray scattering and cross-linking experiments using the cross-linking amino acid p-benzoyl-L-phenylalanine (pBpa). Mutagenesis on residues contributing to the dimer interfaces indicates that there is a link between the dimerization modes and the autocleavage mechanism. We show that for the CTP1L endolysin, there is a reduction in lysis efficiency that is proportional to the cleavage efficiency. We propose a model for endolysin triggering, where the extended dimer presents the inactive state, and a switch to the side-by-side dimer triggers the cleavage of the C-terminal domain. This leads to the release of the catalytic portion of the endolysin, enabling the efficient digestion of the bacterial cell wall.

The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor.,Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R PLoS Pathog. 2014 Jul 24;10(7):e1004228. doi: 10.1371/journal.ppat.1004228., eCollection 2014 Jul. PMID:25058163<ref>PMID:25058163</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4cu5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridium virus phiCD27]]
[[Category: Large Structures]]
[[Category: Dunne M]]
[[Category: Garefalaki V]]
[[Category: Jeffries CM]]
[[Category: Lemke EA]]
[[Category: Mayer MJ]]
[[Category: Meijers R]]
[[Category: Mertens HDT]]
[[Category: Narbad A]]
[[Category: Svergun DI]]
[[Category: Thompson A]]