View source for 4cvu


==Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum==
<StructureSection load='4cvu' size='340' side='right'caption='[[4cvu]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cvu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_harzianum Trichoderma harzianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CVU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cvu OCA], [https://pdbe.org/4cvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cvu RCSB], [https://www.ebi.ac.uk/pdbsum/4cvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cvu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A075B5H6_TRIHA A0A075B5H6_TRIHA] 
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== Publication Abstract from PubMed ==
Hemicellulose is an important part of the plant cell wall biomass relevant to cellulosic ethanol technologies. beta-Mannosidases are enzymes capable of cleavage of non-reducing residues of beta-D-mannose from beta-D-mannosides and hemicellulose mannose-containing polysaccharides, such as mannans and galactomannans. beta-Mannosidases are distributed between glycoside hydrolase (GH) families 1, 2 and 5 with only a handful of the enzymes being structurally characterized to date. The only published X-ray structure of GH2 mannosidase is that of bacterial Bacteroides thetaiotaomicron enzyme. No structures of eukaryotic mannosidases of this family are currently available. To fill this gap we set out to solve the structure of Trichoderma harzianum GH2 beta-mannosidase and to refine it to 1.9 A resolution. Structural comparisons of the T. harzianum GH2 beta-mannosidase highlight similarities in its structural architecture with other members of GH2 family, reveal molecular mechanism of the beta-mannosides binding and recognition and shed light on its putative galactomannan binding site. This article is protected by copyright. All rights reserved.

Insights Into Structure and Function of Fungal beta-mannosidases from Glycoside Hydrolase Family 2 Based on Multiple Crystal Structures of T. harzianum Enzyme.,Nascimento AS, Muniz JR, Aparicio R, Golubev AM, Polikarpov I FEBS J. 2014 Jun 26. doi: 10.1111/febs.12894. PMID:24975648<ref>PMID:24975648</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4cvu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Mannosidase 3D structures|Mannosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trichoderma harzianum]]
[[Category: Aparicio R]]
[[Category: Golubev AM]]
[[Category: Muniz JRC]]
[[Category: Nascimento AS]]
[[Category: Polikarpov I]]
[[Category: Santos JC]]