View source for 4cz9


==Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii at pH 4.==
<StructureSection load='4cz9' size='340' side='right'caption='[[4cz9]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4cz9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi_GE5 Pyrococcus abyssi GE5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CZ9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OOC:OCTYL+4-O-BETA-D-ALLOPYRANOSYL-1-THIO-BETA-D-ALTROPYRANOSIDE'>OOC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cz9 OCA], [https://pdbe.org/4cz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cz9 RCSB], [https://www.ebi.ac.uk/pdbsum/4cz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cz9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9UZ55_PYRAB Q9UZ55_PYRAB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sodium/proton antiporters maintain intracellular pH and sodium levels. Detailed structures of antiporters with bound substrate ions are essential for understanding how they work. We have resolved the substrate ion in the dimeric, electroneutral sodium/proton antiporter PaNhaP from Pyrococcus abyssi at 3.2 A, and have determined its structure in two different conformations at pH 8 and pH 4. The ion is coordinated by three acidic sidechains, a water molecule, a serine and a main-chain carbonyl in the unwound stretch of trans-membrane helix 5 at the deepest point of a negatively charged cytoplasmic funnel. A second narrow polar channel may facilitate proton uptake from the cytoplasm. Transport activity of PaNhaP is cooperative at pH 6 but not at pH 5. Cooperativity is due to pH-dependent allosteric coupling of protomers through two histidines at the dimer interface. Combined with comprehensive transport studies, the structures of PaNhaP offer unique new insights into the transport mechanism of sodium/proton antiporters.

Structure and substrate ion binding in the sodium/proton antiporter PaNhaP.,Wohlert D, Kuhlbrandt W, Yildiz O Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03579. PMID:25426802<ref>PMID:25426802</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4cz9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus abyssi GE5]]
[[Category: Kuhlbrandt W]]
[[Category: Woehlert D]]
[[Category: Yildiz O]]