View source for 4czr


==Crystal structure of the extralong fungal manganese peroxidase from Ceriporiopsis subvermispora in complex with cadmium (anomalous data)==
<StructureSection load='4czr' size='340' side='right'caption='[[4czr]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4czr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gelatoporia_subvermispora Gelatoporia subvermispora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CZR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.984&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4czr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4czr OCA], [https://pdbe.org/4czr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4czr RCSB], [https://www.ebi.ac.uk/pdbsum/4czr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4czr ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The genome of Ceriporiopsis subvermispora includes 13 manganese peroxidase (MnP) genes representative of the three subfamilies described in ligninolytic fungi, which share an Mn(2+)-oxidation site and have varying lengths of the C-terminal tail. Short, long and extralong MnPs were heterologously expressed and biochemically characterized, and the first structure of an extralong MnP was solved. Its C-terminal tail surrounds the haem-propionate access channel, contributing to Mn(2+) oxidation by the internal propionate, but prevents the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), which is only oxidized by short MnPs and by shortened-tail variants from site-directed mutagenesis. The tail, which is anchored by numerous contacts, not only affects the catalytic properties of long/extralong MnPs but is also associated with their high acidic stability. Cd(2+) binds at the Mn(2+)-oxidation site and competitively inhibits oxidation of both Mn(2+) and ABTS. Moreover, mutations blocking the haem-propionate channel prevent substrate oxidation. This agrees with molecular simulations that position ABTS at an electron-transfer distance from the haem propionates of an in silico shortened-tail form, while it cannot reach this position in the extralong MnP crystal structure. Only small differences exist between the long and the extralong MnPs, which do not justify their classification as two different subfamilies, but they significantly differ from the short MnPs, with the presence/absence of the C-terminal tail extension being implicated in these differences.

Structural implications of the C-terminal tail in the catalytic and stability properties of manganese peroxidases from ligninolytic fungi.,Fernandez-Fueyo E, Acebes S, Ruiz-Duenas FJ, Martinez MJ, Romero A, Medrano FJ, Guallar V, Martinez AT Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3253-65. doi:, 10.1107/S1399004714022755. Epub 2014 Nov 22. PMID:25478843<ref>PMID:25478843</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Manganese peroxidase|Manganese peroxidase]]
== References ==
<references/>
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</StructureSection>
[[Category: Gelatoporia subvermispora]]
[[Category: Large Structures]]
[[Category: Medrano FJ]]
[[Category: Romero A]]