View source for 4dzo


==Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting==
<StructureSection load='4dzo' size='340' side='right'caption='[[4dzo]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4dzo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DZO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzo OCA], [https://pdbe.org/4dzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dzo RCSB], [https://www.ebi.ac.uk/pdbsum/4dzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dzo ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The spindle checkpoint prevents aneuploidy by delaying anaphase onset until all sister chromatids achieve proper microtubule attachment. The kinetochore-bound checkpoint protein complex Mad1-Mad2 promotes the conformational activation of Mad2 and serves as a catalytic engine of checkpoint signaling. How Mad1 is targeted to kinetochores is not understood. Here, we report the crystal structure of the conserved C-terminal domain (CTD) of human Mad1. Mad1 CTD forms a homodimer and, unexpectedly, has a fold similar to those of the kinetochore-binding domains of Spc25 and Csm1. Nonoverlapping Mad1 fragments retain detectable kinetochore targeting. Deletion of the CTD diminishes, does not abolish, Mad1 kinetochore localization. Mutagenesis studies further map the functional interface of Mad1 CTD in kinetochore targeting and implicate Bub1 as its receptor. Our results indicate that CTD is a part of an extensive kinetochore-binding interface of Mad1, and rationalize graded kinetochore targeting of Mad1 during checkpoint signaling.

Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting.,Kim S, Sun H, Tomchick DR, Yu H, Luo X Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6549-54. Epub 2012 Apr 9. PMID:22493223<ref>PMID:22493223</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Luo X]]
[[Category: Sun H]]
[[Category: Tomchick DR]]