View source for 4eyz


==Crystal structure of an uncommon cellulosome-related protein module from Ruminococcus flavefaciens that resembles papain-like cysteine peptidases==
<StructureSection load='4eyz' size='340' side='right'caption='[[4eyz]], [[Resolution|resolution]] 1.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4eyz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EYZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.383&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCS:CARBOXYMETHYLATED+CYSTEINE'>CCS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eyz OCA], [https://pdbe.org/4eyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eyz RCSB], [https://www.ebi.ac.uk/pdbsum/4eyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eyz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M9MMP4_RUMFL M9MMP4_RUMFL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: is one of the predominant fiber-degrading bacteria found in the rumen of herbivores. Bioinformatic analysis of the recently sequenced genome indicated that this bacterium produces one of the most intricate cellulosome systems known to date. A distinct ORF, encoding for a multi-modular protein, RflaF_05439, was discovered during mining of the genome sequence. It is composed of two tandem modules of currently undefined function that share 45% identity and a C-terminal X-dockerin modular dyad. Gaining insight into the diversity, architecture and organization of different types of proteins in the cellulosome system is essential for broadening our understanding of a multi-enzyme complex, considered to be one of the most efficient systems for plant cell wall polysaccharide degradation in nature. METHODOLOGYPRINCIPAL FINDINGS: Following bioinformatic analysis, the second tandem module of RflaF_05439 was cloned and its selenium-labeled derivative was expressed and crystallized. The crystals belong to space group P2 with unit-cell parameters of = 65.81, = 60.61, = 66.13 A, beta = 107.66 degrees and contain two protein molecules in the asymmetric unit. The crystal structure was determined at 1.38-A resolution by X-ray diffraction using the single-wavelength anomalous dispersion (SAD) method and was refined to R and R of 0.127 and 0.152 respectively. The protein molecule mainly comprises a beta-sheet flanked by short alpha-helixes, and a globular alpha-helical domain. The structure was found to be structurally similar to members of the NlpC/P60 superfamily of cysteine peptidases. CONCLUSIONSSIGNIFICANCE: The 3D structure of the second repeat of the RflaF_05439 enabled us to propose a role for the currently undefined function of this protein. Its putative function as a cysteine peptidase is inferred from structural homology studies. It is therefore apparent that cellulosomes integrate proteins with other functions in addition to the classic well-defined carbohydrate active enzymes.

Crystal Structure of an Uncommon Cellulosome-Related Protein Module from Ruminococcus flavefaciens That Resembles Papain-Like Cysteine Peptidases.,Levy-Assaraf M, Voronov-Goldman M, Rozman Grinberg I, Weiserman G, Shimon LJ, Jindou S, Borovok I, White BA, Bayer EA, Lamed R, Frolow F PLoS One. 2013;8(2):e56138. doi: 10.1371/journal.pone.0056138. Epub 2013 Feb 14. PMID:23457513<ref>PMID:23457513</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4eyz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ruminococcus flavefaciens]]
[[Category: Bayer E]]
[[Category: Frolow F]]
[[Category: Lamed R]]
[[Category: Voronov-Goldman M]]