View source for 4fhf


==Spore photoproduct lyase C140A mutant with dinucleoside spore photoproduct==
<StructureSection load='4fhf' size='340' side='right'caption='[[4fhf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4fhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FHF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0TT:1-[(2R,4S,5R)-5-(HYDROXYMETHYL)-4-OXIDANYL-OXOLAN-2-YL]-5-[[(5R)-1-[(2R,4S,5R)-5-(HYDROXYMETHYL)-4-OXIDANYL-OXOLAN-2-YL]-5-METHYL-2,4-BIS(OXIDANYLIDENE)-1,3-DIAZINAN-5-YL]METHYL]PYRIMIDINE-2,4-DIONE'>0TT</scene>, <scene name='pdbligand=EEM:[(3S)-3-AMINO-4-HYDROXY-4-OXO-BUTYL]-[[(2S,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL]-METHYL-SELANIUM'>EEM</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fhf OCA], [https://pdbe.org/4fhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fhf RCSB], [https://www.ebi.ac.uk/pdbsum/4fhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fhf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4IQU1_GEOTN A4IQU1_GEOTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial spores possess an enormous resistance to ultraviolet (UV) radiation. This is largely due to a unique DNA repair enzyme, Spore Photoproduct Lyase (SP lyase) that repairs a specific UV-induced DNA lesion, the spore photoproduct (SP), through an unprecedented radical-based mechanism. Unlike DNA photolyases, SP lyase belongs to the emerging superfamily of radical S-adenosyl-l-methionine (SAM) enzymes and uses a [4Fe-4S](1+) cluster and SAM to initiate the repair reaction. We report here the first crystal structure of this enigmatic enzyme in complex with its [4Fe-4S] cluster and its SAM cofactor, in the absence and presence of a DNA lesion, the dinucleoside SP. The high resolution structures provide fundamental insights into the active site, the DNA lesion recognition and binding which involve a beta-hairpin structure. We show that SAM and a conserved cysteine residue are perfectly positioned in the active site for hydrogen atom abstraction from the dihydrothymine residue of the lesion and donation to the alpha-thyminyl radical moiety, respectively. Based on structural and biochemical characterizations of mutant proteins, we substantiate the role of this cysteine in the enzymatic mechanism. Our structure reveals how SP lyase combines specific features of radical SAM and DNA repair enzymes to enable a complex radical-based repair reaction to take place.

Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme.,Benjdia A, Heil K, Barends TR, Carell T, Schlichting I Nucleic Acids Res. 2012 Jul 2. PMID:22761404<ref>PMID:22761404</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4fhf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus thermodenitrificans NG80-2]]
[[Category: Large Structures]]
[[Category: Barends TRM]]
[[Category: Benjdia A]]
[[Category: Carell T]]
[[Category: Heil K]]
[[Category: Schlichting I]]