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==Crystal Structure of SsfS6, Streptomyces sp. SF2575 glycosyltransferase==
<StructureSection load='4fzr' size='340' side='right'caption='[[4fzr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4fzr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SF2575 Streptomyces sp. SF2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.397&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzr OCA], [https://pdbe.org/4fzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzr RCSB], [https://www.ebi.ac.uk/pdbsum/4fzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzr ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc.

Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584<ref>PMID:23526584</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sp. SF2575]]
[[Category: Bingman CA]]
[[Category: Phillips Jr GN]]
[[Category: Singh S]]
[[Category: Thorson JS]]
[[Category: Wang F]]
[[Category: Zhou M]]