View source for 4gey


==High pH structure of Pseudomonas putida OprB==
<StructureSection load='4gey' size='340' side='right'caption='[[4gey]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gey]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_BIRD-1 Pseudomonas putida BIRD-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gey OCA], [https://pdbe.org/4gey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gey RCSB], [https://www.ebi.ac.uk/pdbsum/4gey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gey ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A5VZA8_PSEP1 A5VZA8_PSEP1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Substrate-specific outer membrane (OM) channels of Gram-negative bacteria mediate uptake of many small molecules, including carbohydrates. The mechanism of sugar uptake by enterobacterial channels such as E. coli LamB (maltoporin) has been characterized in great detail. In pseudomonads and related organisms, sugar uptake is not mediated by LamB, but by OprB channels. Beyond the notion that OprB channels seem to prefer monosaccharides as substrates, very little is known about OprB-mediated sugar uptake. Here I report the X-ray crystal structure of an OprB channel from Pseudomonas putida F1. The structure shows that OprB forms a monomeric, 16-stranded beta-barrel with a constriction formed by extracellular loops L2 and L3. The side chains of two highly conserved arginine residues (Arg83 and Arg110) and a conserved glutamate (Glu106) line the channel constriction and interact with a bound glucose molecule. Liposome swelling uptake assays show a strong preference for monosaccharide transport over disaccharides. Moreover, substrates with a net negative charge are disfavored by the channel, likely due to the negatively charged character of the constriction. The architecture of the eyelet and the absence of a greasy slide provide an explanation for the observed specificity of OprB for monosaccharides rather than the oligosaccharides preferred by LamB and related enterobacterial channels.

Structural basis for outer membrane sugar uptake in pseudomonads.,van den Berg B J Biol Chem. 2012 Oct 12. PMID:23066028<ref>PMID:23066028</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gey" style="background-color:#fffaf0;"></div>

==See Also==
*[[Porin 3D structures|Porin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas putida BIRD-1]]
[[Category: Van den Berg B]]