View source for 4gqf


==Aeropyrum pernix Peroxiredoxin Q Enzyme in the Locally Unfolded Conformation==
<StructureSection load='4gqf' size='340' side='right'caption='[[4gqf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gqf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GQF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gqf OCA], [https://pdbe.org/4gqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gqf RCSB], [https://www.ebi.ac.uk/pdbsum/4gqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gqf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9YA14_AERPE Q9YA14_AERPE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peroxiredoxins (Prx) make up a family of enzymes that reduce peroxides using a peroxidatic cysteine residue; among these, members of the PrxQ subfamily are proposed to be the most ancestral-like yet are among the least characterized. In many PrxQ enzymes, a second "resolving" cysteine is located five residues downstream from the peroxidatic Cys, and these residues form a disulfide during the catalytic cycle. Here, we describe three hyperthermophilic PrxQ crystal structures originally determined by the RIKEN structural genomics group. We reprocessed the diffraction data and conducted further refinement to yield models with R(free) values lowered by 2.3-7.2% and resolution extended by 0.2-0.3 A, making one, at 1.4 A, one of the best resolved peroxiredoxins to date. Comparisons of two matched thiol and disulfide forms reveal that the active site conformational change required for disulfide formation involves a transition of approximately 20 residues from a pair of alpha-helices to a beta-hairpin and 3(10)-helix. Each conformation has approximately 10 residues with a high level of disorder providing slack that allows the dramatic shift, and the two conformations are anchored to the protein core by distinct nonpolar side chains that fill three hydrophobic pockets. Sequence conservation patterns confirm the importance of these and a few additional residues for function. From a broader perspective, this study raises the provocative question of how to make use of the valuable information in the Protein Data Bank generated by structural genomics projects but not described in the literature, perhaps remaining unrecognized and certainly underutilized.

Mapping the Active Site Helix-to-Strand Conversion of CxxxxC Peroxiredoxin Q Enzymes.,Perkins A, Gretes MC, Nelson KJ, Poole LB, Karplus PA Biochemistry. 2012 Sep 14. PMID:22928725<ref>PMID:22928725</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gqf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacterioferritin comigratory protein|Bacterioferritin comigratory protein]]
*[[Thiol peroxidase 3D structures|Thiol peroxidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aeropyrum pernix K1]]
[[Category: Large Structures]]
[[Category: Gretes MC]]
[[Category: Karplus PA]]
[[Category: Nelson KJ]]
[[Category: Perkins A]]
[[Category: Poole LB]]