View source for 4gvb


==Crystal structure of the virally encoded antifungal protein, KP6, heterodimer==
<StructureSection load='4gvb' size='340' side='right'caption='[[4gvb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gvb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ustilago_maydis_virus_P6 Ustilago maydis virus P6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GVB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gvb OCA], [https://pdbe.org/4gvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gvb RCSB], [https://www.ebi.ac.uk/pdbsum/4gvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gvb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KP6T_UMV6 KP6T_UMV6] This protein is lethal to sensitive cells of the same or related species. The KP6 alpha subunit is known to recognize some cellular receptors before interaction of the complex with KP6 beta, precipitating cell death.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Killer toxins are produced by several genera of yeast and filamentous fungi. A small proportion of Ustilago maydis strains produce killer toxins, to which they are resistant, but sensitive strains are the majority in the wild populations. There are three killer types (P1, P4 and P6) that secrete KP1, KP4 and KP6 toxins, respectively, which are produced only by strains persistently infected with double-stranded RNA viruses (UmV) in the cell cytoplasm. Unlike nearly all other viruses, UmV are only transmitted through mitosis or meiosis. As shown here, KP6 is different from any other known cytotoxic protein. KP6 is neutral protein composed of two subunits: KP6alpha and KP6beta. KP6alpha is responsible for targeting while KP6beta is cytotoxic. Neither subunit is homologous in either sequence or structure to any other toxin, but they have highly similar structures to each other. The major difference between the two subunits is a hydrophobic helix at the N-terminus of KP6alpha and is likely key to target recognition. Unlike any other toxin, KP6 is translated as a single polypeptide with a 31-residue linker region in the middle of the protein. From structural prediction studies, this linker likely makes for a more compact KP6 structure that sequesters the hydrophobic helix of KP6alpha. A model whereby the protoxin undergoes a conformational activation process that exposes this helix immediately prior to secretion is presented.

The Atomic Structure of the Virally Encoded Antifungal Protein, KP6.,Allen A, Chatt E, Smith TJ J Mol Biol. 2012 Dec 3. pii: S0022-2836(12)00905-9. doi:, 10.1016/j.jmb.2012.11.033. PMID:23219466<ref>PMID:23219466</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gvb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ustilago maydis virus P6]]
[[Category: Smith T]]