View source for 4gyc


==Structure of the SRII(D75N mutant)/HtrII Complex in I212121 space group ("U" shape)==
<StructureSection load='4gyc' size='340' side='right'caption='[[4gyc]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gyc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GYC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.0501&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyc OCA], [https://pdbe.org/4gyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gyc RCSB], [https://www.ebi.ac.uk/pdbsum/4gyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gyc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACS2_NATPH BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.

Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer.,Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Balandin T, Remeeva A, Engelhard M, Buldt G, Gordeliy V J Photochem Photobiol B. 2013 Jun 5;123:55-8. doi:, 10.1016/j.jphotobiol.2013.03.008. Epub 2013 Mar 29. PMID:23619282<ref>PMID:23619282</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gyc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Natronomonas pharaonis]]
[[Category: Balandin T]]
[[Category: Borshchevskiy V]]
[[Category: Bueldt G]]
[[Category: Engelhard M]]
[[Category: Gordeliy V]]
[[Category: Grudinin S]]
[[Category: Gushchin I]]
[[Category: Ishchenko A]]
[[Category: Klare J]]
[[Category: Remeeva A]]
[[Category: Round E]]
[[Category: Utrobin P]]