View source for 4h3u


==Crystal structure of hypothetical protein with ketosteroid isomerase-like protein fold from Catenulispora acidiphila DSM 44928==
<StructureSection load='4h3u' size='340' side='right'caption='[[4h3u]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4h3u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catenulispora_acidiphila_DSM_44928 Catenulispora acidiphila DSM 44928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3u OCA], [https://pdbe.org/4h3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3u RCSB], [https://www.ebi.ac.uk/pdbsum/4h3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3u ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Catenulispora acidiphila is a newly identified lineage of actinomycetes that produces antimicrobial activities and represents a promising source of novel antibiotics and secondary metabolites. Among the discovered protein coding genes, 68 % were assigned a putative function, while the remaining 32 % are genes encoding "hypothetical" proteins. Caci_0382 is one of the "hypothetical" proteins that has very few homologs. Sequence analysis shows that the protein belongs to the NTF2-like protein family. The structure of Caci_0382 demonstrates that it shares the same fold and has a similar active site as limonene-1,2-epoxide hydrolase, which suggests that it may have a related function. Using a fluorescence thermal shift assay, we identified stabilizing compounds that suggest potential natural ligands of Caci_0382. Using this information, we determined the crystal structure in complex with trimethylamine to provide a better understanding of the function of this uncharacterized protein.

Structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in Catenulispora acidiphila.,Filippova EV, Luan CH, Dunne SF, Kiryukhina O, Minasov G, Shuvalova L, Anderson WF J Struct Funct Genomics. 2014 Mar;15(1):33-40. doi: 10.1007/s10969-014-9176-z., Epub 2014 Feb 22. PMID:24562475<ref>PMID:24562475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4h3u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Catenulispora acidiphila DSM 44928]]
[[Category: Large Structures]]
[[Category: Anderson WF]]
[[Category: Filippova EV]]
[[Category: Jedrzejczak R]]
[[Category: Joachimiak A]]
[[Category: Kiryukhina O]]
[[Category: Minasov G]]
[[Category: Shuvalova L]]