View source for 4hqf


==Crystal structure of Plasmodium falciparum TRAP, I4 form==
<StructureSection load='4hqf' size='340' side='right'caption='[[4hqf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HQF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hqf OCA], [https://pdbe.org/4hqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hqf RCSB], [https://www.ebi.ac.uk/pdbsum/4hqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hqf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q76NM2_PLAF7 Q76NM2_PLAF7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sporozoite gliding motility and invasion of mosquito and vertebrate host cells in malaria is mediated by thrombospondin repeat anonymous protein (TRAP). Tandem von Willebrand factor A (VWA) and thrombospondin type I repeat (TSR) domains in TRAP connect through proline-rich stalk, transmembrane, and cytoplasmic domains to the parasite actin-dependent motility apparatus. We crystallized fragments containing the VWA and TSR domains from Plasmodium vivax and Plasmodium falciparum in different crystal lattices. TRAP VWA domains adopt closed and open conformations, and bind a Mg(2+) ion at a metal ion-dependent adhesion site implicated in ligand binding. Metal ion coordination in the open state is identical to that seen in the open high-affinity state of integrin I domains. The closed VWA conformation associates with a disordered TSR domain. In contrast, the open VWA conformation crystallizes with an extensible beta ribbon and ordered TSR domain. The extensible beta ribbon is composed of disulfide-bonded segments N- and C-terminal to the VWA domain that are largely drawn out of the closed VWA domain in a 15 A movement to the open conformation. The extensible beta ribbon and TSR domain overlap at a conserved interface. The VWA, extensible beta ribbon, and TSR domains adopt a highly elongated overall orientation that would be stabilized by tensile force exerted across a ligand-receptor complex by the actin motility apparatus of the sporozoite. Our results provide insights into regulation of "stick-and-slip" parasite motility and for development of sporozoite subunit vaccines.

Shape change in the receptor for gliding motility in Plasmodium sporozoites.,Song G, Koksal AC, Lu C, Springer TA Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21420-5. doi:, 10.1073/pnas.1218581109. Epub 2012 Dec 11. PMID:23236185<ref>PMID:23236185</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hqf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium falciparum 3D7]]
[[Category: Koksal AC]]
[[Category: Lu C]]
[[Category: Song G]]
[[Category: Springer TA]]