View source for 4hzb


==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==
<StructureSection load='4hzb' size='340' side='right'caption='[[4hzb]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_pickettii_12D Ralstonia pickettii 12D]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [https://pdbe.org/4hzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [https://www.ebi.ac.uk/pdbsum/4hzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C6BHF2_RALP1 C6BHF2_RALP1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recently described T6SS (type VI secretion system) acts as a needle that punctures the membrane of the target cells to deliver effector proteins. Type VI amidase effectors can be classified into four divergent families (Tae1-Tae4). These effectors are secreted into the periplasmic space of neighbouring cells via the T6SS and subsequently rupture peptidoglycan. However, the donor cells are protected from damage because of the presence of their cognate immunity proteins [Tai1 (type VI amidase immunity 1)-Tai4]. In the present paper, we describe the structure of Tae3 in complex with Tai3. The Tae3-Tai3 complex exists as a stable heterohexamer, which is composed of two Tae3 molecules and two Tai3 homodimers (Tae3-Tai34-Tae3). Tae3 shares a common NlpC/P60 fold, which consists of N-terminal and C-terminal subdomains. Structural analysis indicates that two unique loops around the catalytic cleft adopt a closed conformation, resulting in a narrow and extended groove involved in the binding of the substrate. The inhibition of Tae3 is attributed to the insertion of the Omega-loop (loop of alpha3-alpha4) of Tai3 into the catalytic groove. Furthermore, a cell viability assay confirmed that a conserved motif (Gln-Asp-Xaa) in Tai3 members may play a key role in the inhibition process. Taken together, the present study has revealed a novel inhibition mechanism and provides insights into the role played by T6SS in interspecific competition.

Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3.,Dong C, Zhang H, Gao ZQ, Wang WJ, She Z, Liu GF, Shen YQ, Su XD, Dong YH Biochem J. 2013 Aug 15;454(1):59-68. doi: 10.1042/BJ20130193. PMID:23730712<ref>PMID:23730712</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hzb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Ralstonia pickettii 12D]]
[[Category: Dong C]]
[[Category: Dong YH]]
[[Category: Gao ZQ]]
[[Category: Zhang H]]