View source for 4i1s


==Melanoma differentiation associated protein-5 Helicase domain complex with inhibitor Non-structural protein V==
<StructureSection load='4i1s' size='340' side='right'caption='[[4i1s]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4i1s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_virus_5_(strain_W3) Simian virus 5 (strain W3)] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I1S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.293&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i1s OCA], [https://pdbe.org/4i1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i1s RCSB], [https://www.ebi.ac.uk/pdbsum/4i1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i1s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A7LCX1_PIG A7LCX1_PIG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RIG-I like receptor MDA5 senses cytoplasmic viral RNA and activates antiviral innate immunity. To reveal how paramyxoviruses counteract this response, we determined the crystal structure of the MDA5 ATP-hydrolysis domain in complex with the viral inhibitor V protein. The V protein unfolded the ATPase domain of MDA5 via a beta-hairpin motif and recognized a structural motif of MDA5 that is normally buried in the conserved helicase fold. This leads to disruption of the MDA5 ATP-hydrolysis site and prevention of RNA bound MDA5 filament formation. The structure explains why V proteins inactivate MDA5, but not RIG-I, and mutating only two amino acids in RIG-I induces robust V protein binding. Our results suggest an inhibition mechanism of RLR signalosome formation by unfolding of receptor and inhibitor.

Paramyxovirus V Proteins Disrupt the Fold of the RNA Sensor MDA5 to Inhibit Antiviral Signaling.,Motz C, Schuhmann KM, Kirchhofer A, Moldt M, Witte G, Conzelmann KK, Hopfner KP Science. 2013 Jan 17. PMID:23328395<ref>PMID:23328395</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4i1s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Hopfner KP]]
[[Category: Motz C]]
[[Category: Witte G]]