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==Crystal structure of SplD protease from Staphylococcus aureus at 1.56 A resolution==
<StructureSection load='4ink' size='340' side='right'caption='[[4ink]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ink]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4INK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4INK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ink FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ink OCA], [https://pdbe.org/4ink PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ink RCSB], [https://www.ebi.ac.uk/pdbsum/4ink PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ink ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPLD_STAA8 SPLD_STAA8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcus aureus is a dangerous human pathogen. A number of the proteins secreted by this bacterium are implicated in its virulence, but many of the components of its secretome are poorly characterized. Strains of S. aureus can produce up to six homologous extracellular serine proteases grouped in a single spl operon. Although the SplA, SplB, and SplC proteases have been thoroughly characterized, the properties of the other three enzymes have not yet been investigated. Here, we describe the biochemical and structural characteristics of the SplD protease. The active enzyme was produced in an Escherichia coli recombinant system and purified to homogeneity. P1 substrate specificity was determined using a combinatorial library of synthetic peptide substrates showing exclusive preference for threonine, serine, leucine, isoleucine, alanine, and valine. To further determine the specificity of SplD, we used high-throughput synthetic peptide and cell surface protein display methods. The results not only confirmed SplD preference for a P1 residue, but also provided insight into the specificity of individual primed- and non-primed substrate-binding subsites. The analyses revealed a surprisingly narrow specificity of the protease, which recognized five consecutive residues (P4-P3-P2-P1-P1') with a consensus motif of R-(Y/W)-(P/L)-(T/L/I/V) downward arrowS. To understand the molecular basis of the strict substrate specificity, we crystallized the enzyme in two different conditions, and refined the structures at resolutions of 1.56 A and 2.1 A. Molecular modeling and mutagenesis studies allowed us to define a consensus model of substrate binding, and illustrated the molecular mechanism of protease specificity.

Biochemical and Structural Characterization of SplD Protease from Staphylococcus aureus.,Zdzalik M, Kalinska M, Wysocka M, Stec-Niemczyk J, Cichon P, Stach N, Gruba N, Stennicke HR, Jabaiah A, Markiewicz M, Kedracka-Krok S, Wladyka B, Daugherty PS, Lesner A, Rolka K, Dubin A, Potempa J, Dubin G PLoS One. 2013 Oct 9;8(10):e76812. doi: 10.1371/journal.pone.0076812. PMID:24130791<ref>PMID:24130791</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ink" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
[[Category: Cichon P]]
[[Category: Daugherty PS]]
[[Category: Dubin A]]
[[Category: Dubin G]]
[[Category: Jabaiah A]]
[[Category: Kalinska M]]
[[Category: Lesner A]]
[[Category: Markiewicz M]]
[[Category: Potempa J]]
[[Category: Rolka K]]
[[Category: Stec-Niemczyk J]]
[[Category: Stennicke HR]]
[[Category: Wladyka B]]
[[Category: Wysocka M]]
[[Category: Zdzalik M]]