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==Crystal Structure of the Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii (in P21 form)==
<StructureSection load='4iwm' size='340' side='right'caption='[[4iwm]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4iwm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IWM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iwm OCA], [https://pdbe.org/4iwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iwm RCSB], [https://www.ebi.ac.uk/pdbsum/4iwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iwm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCH1L_METJA GCH1L_METJA] DNA-binding protein exhibiting the ability to bind to both single-stranded and double-stranded DNA.<ref>PMID:25243119</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A Nif3 family protein of Methanocaldococcus jannaschii, MJ0927, is highly conserved from bacteria to humans. Although several structures of bacterial Nif3 proteins are known, no structure representing archaeal Nif3 has yet been reported. The crystal structure of Methanocaldococcus jannaschii MJ0927 was determined at 2.47 A resolution to understand the structural differences between the bacterial and archaeal Nif3 proteins. Intriguingly, MJ0927 is found to adopt an unusual assembly comprising a trimer of dimers that forms a cage-like architecture. Electrophoretic mobility-shift assays indicate that MJ0927 binds to both single-stranded and double-stranded DNA. Structural analysis of MJ0927 reveals a positively charged region that can potentially explain its DNA-binding capability. Taken together, these data suggest that MJ0927 adopts a novel quartenary architecture that could play various DNA-binding roles in Methanocaldococcus jannaschii.

Crystal structure of a conserved hypothetical protein MJ0927 from Methanocaldococcus jannaschii reveals a novel quaternary assembly in the Nif3 family.,Chen SC, Huang CH, Yang CS, Kuan SM, Lin CT, Chou SH, Chen Y Biomed Res Int. 2014;2014:171263. doi: 10.1155/2014/171263. Epub 2014 Aug 28. PMID:25243119<ref>PMID:25243119</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4iwm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii DSM 2661]]
[[Category: Chen SC]]
[[Category: Chen Y]]
[[Category: Chen YR]]
[[Category: Kuan SM]]
[[Category: Liu YH]]
[[Category: Yang CS]]