View source for 4izl


==Structure Of The N248A Mutant of the PANTON-VALENTINE LEUCOCIDIN S Component from STAPHYLOCOCCUS AUREUS==
<StructureSection load='4izl' size='340' side='right'caption='[[4izl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4izl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_phage_PVL Staphylococcus phage PVL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IZL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4izl OCA], [https://pdbe.org/4izl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4izl RCSB], [https://www.ebi.ac.uk/pdbsum/4izl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4izl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O80066_9CAUD O80066_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Panton-Valentine leukocidin (PVL), a bicomponent staphylococcal leukotoxin, is involved in the poor prognosis of necrotizing pneumonia. The present study aimed to elucidate the binding mechanism of PVL and in particular its cell-binding domain. The class S component of PVL, LukS-PV, is known to ensure cell targeting and exhibits the highest affinity for the neutrophil membrane (Kd approximately 10-10 M) compared to the class F component of PVL, LukF-PV (Kd approximately 10-9 M). Alanine scanning mutagenesis was used to identify the residues involved in LukS-PV binding to the neutrophil surface. Nineteen single alanine mutations were performed in the rim domain previously described as implicated in cell membrane interactions. Positions were chosen in order to replace polar or exposed charged residues and according to conservation between leukotoxin class S components. Characterization studies enabled to identify a cluster of residues essential for LukS-PV binding, localized on two loops of the rim domain. The mutations R73A, Y184A, T244A, H245A and Y250A led to dramatically reduced binding affinities for both human leukocytes and undifferentiated U937 cells expressing the C5a receptor. The three-dimensional structure of five of the mutants was determined using X-ray crystallography. Structure analysis identified residues Y184 and Y250 as crucial in providing structural flexibility in the receptor-binding domain of LukS-PV.

Residues essential for panton-valentine leukocidin s component binding to its cell receptor suggest both plasticity and adaptability in its interaction surface.,Laventie BJ, Guerin F, Mourey L, Tawk MY, Jover E, Maveyraud L, Prevost G PLoS One. 2014 Mar 18;9(3):e92094. doi: 10.1371/journal.pone.0092094. eCollection, 2014. PMID:24643034<ref>PMID:24643034</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4izl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus phage PVL]]
[[Category: Laventie BJ]]
[[Category: Maveyraud L]]
[[Category: Mourey L]]
[[Category: Prevost G]]