View source for 4j01


==Crystal Structure of Fischerella Transcription Factor HetR complexed with 29mer DNA target==
<StructureSection load='4j01' size='340' side='right'caption='[[4j01]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4j01]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena Anabaena] and [https://en.wikipedia.org/wiki/Fischerella_thermalis_PCC_7521 Fischerella thermalis PCC 7521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J01 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.246&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j01 OCA], [https://pdbe.org/4j01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j01 RCSB], [https://www.ebi.ac.uk/pdbsum/4j01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j01 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2ACK9_9CYAN Q2ACK9_9CYAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-A resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date.

Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.,Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A Proc Natl Acad Sci U S A. 2013 Apr 22. PMID:23610410<ref>PMID:23610410</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4j01" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Anabaena]]
[[Category: Fischerella thermalis PCC 7521]]
[[Category: Large Structures]]
[[Category: Gornicki P]]
[[Category: Joachimiak A]]
[[Category: Joachimiak G]]
[[Category: Kim Y]]
[[Category: MCSG]]