View source for 4jgz


==Crystal structure of human coxsackievirus A16 uncoating intermediate (space group I222)==
<StructureSection load='4jgz' size='340' side='right'caption='[[4jgz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4jgz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_A16 Coxsackievirus A16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JGZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jgz OCA], [https://pdbe.org/4jgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jgz RCSB], [https://www.ebi.ac.uk/pdbsum/4jgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jgz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I3W9E1_9ENTO I3W9E1_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611]  Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266]  RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
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== Publication Abstract from PubMed ==
It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release.

Picornavirus uncoating intermediate captured in atomic detail.,Ren J, Wang X, Hu Z, Gao Q, Sun Y, Li X, Porta C, Walter TS, Gilbert RJ, Zhao Y, Axford D, Williams M, McAuley K, Rowlands DJ, Yin W, Wang J, Stuart DI, Rao Z, Fry EE Nat Commun. 2013 Jun 3;4:1929. doi: 10.1038/ncomms2889. PMID:23728514<ref>PMID:23728514</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4jgz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Coxsackievirus A16]]
[[Category: Large Structures]]
[[Category: Axford D]]
[[Category: Fry EE]]
[[Category: Gao Q]]
[[Category: Gilbert RJ]]
[[Category: Hu Z]]
[[Category: Li X]]
[[Category: McAuley K]]
[[Category: Porta C]]
[[Category: Rao Z]]
[[Category: Ren J]]
[[Category: Rowlands DJ]]
[[Category: Stuart DI]]
[[Category: Sun Y]]
[[Category: Walter TS]]
[[Category: Wang J]]
[[Category: Wang X]]
[[Category: Williams M]]
[[Category: Yin W]]
[[Category: Zhao Y]]