View source for 4jie


==Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase==
<StructureSection load='4jie' size='340' side='right'caption='[[4jie]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4jie]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Indica_Group Oryza sativa Indica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JIE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jie OCA], [https://pdbe.org/4jie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jie RCSB], [https://www.ebi.ac.uk/pdbsum/4jie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jie ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2YPH1_ORYSI A2YPH1_ORYSI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rice Os7BGlu26 is a GH1 family glycoside hydrolase with a threefold higher kcat/Km value for 4-nitrophenyl beta-D-mannoside (4NPMan) compared with 4-nitrophenyl beta-D-glucoside (4NPGlc). To investigate its selectivity for beta-D-mannoside and beta-D-glucoside substrates, the structures of apo Os7BGlu26 at a resolution of 2.20 A and of Os7BGlu26 with mannose at a resolution of 2.45 A were elucidated from isomorphous crystals in space group P212121. The (beta/alpha)8-barrel structure is similar to other GH1 family structures, but with a narrower active-site cleft. The Os7BGlu26 structure with D-mannose corresponds to a product complex, with beta-D-mannose in the (1)S5 skew-boat conformation. Docking of the (1)S3, (1)S5, (2)SO and (3)S1 pyranose-ring conformations of 4NPMan and 4NPGlc substrates into the active site of Os7BGlu26 indicated that the lowest energies were in the (1)S5 and (1)S3 skew-boat conformations. Comparison of these docked conformers with other rice GH1 structures revealed differences in the residues interacting with the catalytic acid/base between enzymes with and without beta-D-mannosidase activity. The mutation of Tyr134 to Trp in Os7BGlu26 resulted in similar kcat/Km values for 4NPMan and 4NPGlc, while mutation of Tyr134 to Phe resulted in a 37-fold higher kcat/Km for 4NPMan than 4NPGlc. Mutation of Cys182 to Thr decreased both the activity and the selectivity for beta-D-mannoside. It was concluded that interactions with the catalytic acid/base play a significant role in glycon selection.

Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase.,Tankrathok A, Iglesias-Fernandez J, Luang S, Robinson RC, Kimura A, Rovira C, Hrmova M, Ketudat Cairns JR Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):2124-35. doi:, 10.1107/S0907444913020568. Epub 2013 Sep 20. PMID:24100330<ref>PMID:24100330</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4jie" style="background-color:#fffaf0;"></div>

==See Also==
*[[Mannosidase|Mannosidase]]
*[[Mannosidase 3D structures|Mannosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Indica Group]]
[[Category: Hrmova M]]
[[Category: Ketudat Cairns JR]]
[[Category: Kimura A]]
[[Category: Luang S]]
[[Category: Robinson RC]]
[[Category: Tankrathok A]]