View source for 4jjf


==Crystal structure of FE-hydrogenase from methanothermobacter marburgensis in complex with 2-naphthylisocyanide==
<StructureSection load='4jjf' size='340' side='right'caption='[[4jjf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4jjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_marburgensis_str._Marburg Methanothermobacter marburgensis str. Marburg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=N2I:N-(NAPHTHALEN-2-YL)METHANIMINE'>N2I</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjf OCA], [https://pdbe.org/4jjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jjf RCSB], [https://www.ebi.ac.uk/pdbsum/4jjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HMD_METTM HMD_METTM] Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Inhibition mechanism: Isocyanides strongly inhibit [Fe]-hydrogenase. X-ray crystallography and X-ray absorption spectroscopy revealed that the isocyanide binds to the trans position, versus the acyl carbon of the Fe center, and is covalently bound to the pyridinol hydroxy oxygen. These results also indicated that the hydroxy group is essential for H2 activation.

Crystal Structures of [Fe]-Hydrogenase in Complex with Inhibitory Isocyanides: Implications for the H -Activation Site.,Tamura H, Salomone-Stagni M, Fujishiro T, Warkentin E, Meyer-Klaucke W, Ermler U, Shima S Angew Chem Int Ed Engl. 2013 Jul 22. doi: 10.1002/anie.201305089. PMID:23873755<ref>PMID:23873755</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4jjf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanothermobacter marburgensis str. Marburg]]
[[Category: Ermler U]]
[[Category: Shima S]]
[[Category: Tamura H]]
[[Category: Warkentin E]]