View source for 4kki


==Crystal Structure of Haptocorrin in Complex with CNCbl==
<StructureSection load='4kki' size='340' side='right'caption='[[4kki]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kki]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KKI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CNC:CO-CYANOCOBALAMIN'>CNC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kki OCA], [https://pdbe.org/4kki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kki RCSB], [https://www.ebi.ac.uk/pdbsum/4kki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kki ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TCO1_HUMAN TCO1_HUMAN] Vitamin B12-binding protein. Transports cobalamin into cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cobalamin (Cbl; vitamin B12) is an essential micronutrient synthesized only by bacteria. Mammals have developed a sophisticated uptake system to capture the vitamin from the diet. Cbl transport is mediated by three transport proteins: transcobalamin, intrinsic factor, and haptocorrin (HC). All three proteins have a similar overall structure but a different selectivity for corrinoids. Here, we present the crystal structures of human HC in complex with cyanocobalamin and cobinamide at 2.35 and 3.0 A resolution, respectively. The structures reveal that many of the interactions with the corrin ring are conserved among the human Cbl transporters. However, the non-conserved residues Asn-120, Arg-357, and Asn-373 form distinct interactions allowing for stabilization of corrinoids other than Cbl. A central binding motif forms interactions with the e- and f-side chains of the corrin ring and is conserved in corrinoid-binding proteins of other species. In addition, the alpha- and beta-domains of HC form several unique interdomain contacts and have a higher shape complementarity than those of intrinsic factor and transcobalamin. The stabilization of ligands by all of these interactions is reflected in higher melting temperatures of the protein-ligand complexes. Our structural analysis offers fundamental insights into the unique binding behavior of HC and completes the picture of Cbl interaction with its three transport proteins.

Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin.,Furger E, Frei DC, Schibli R, Fischer E, Prota AE J Biol Chem. 2013 Aug 30;288(35):25466-76. doi: 10.1074/jbc.M113.483271. Epub, 2013 Jul 11. PMID:23846701<ref>PMID:23846701</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4kki" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fischer E]]
[[Category: Frei DC]]
[[Category: Furger E]]
[[Category: Prota AE]]
[[Category: Schibli R]]