View source for 4kkt


==Crystal Structure of BesA (P21 form)==
<StructureSection load='4kkt' size='340' side='right'caption='[[4kkt]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kkt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KKT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kkt OCA], [https://pdbe.org/4kkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kkt RCSB], [https://www.ebi.ac.uk/pdbsum/4kkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kkt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O51166_BORBU O51166_BORBU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35A resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-domain architecture evident among proteobacteria and retains the lipoyl, beta-barrel and membrane-proximal domains that interact with the periplasmic domains of the inner membrane transporter. However, it lacks the alpha-hairpin domain shown to establish extensive coiled-coil interactions with the periplasmic entrance helices of the outer membrane-anchored TolC exit duct. This has implications for the modelling of assembled tripartite efflux pumps.

Structure of an atypical periplasmic adaptor from a multidrug efflux pump of the spirochete Borrelia burgdorferi.,Greene NP, Hinchliffe P, Crow A, Ababou A, Hughes C, Koronakis V FEBS Lett. 2013 Jul 9. pii: S0014-5793(13)00520-6. doi:, 10.1016/j.febslet.2013.06.056. PMID:23851070<ref>PMID:23851070</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4kkt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Borreliella burgdorferi B31]]
[[Category: Large Structures]]
[[Category: Ababou A]]
[[Category: Crow A]]
[[Category: Greene NP]]
[[Category: Hinchliffe P]]
[[Category: Hughes C]]
[[Category: Koronakis V]]