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==Structure of the Fab fragment of N62, a protective monoclonal antibody to the nonreducing end of Francisella tularensis O-antigen==
<StructureSection load='4kph' size='340' side='right'caption='[[4kph]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kph]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kph OCA], [https://pdbe.org/4kph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kph RCSB], [https://www.ebi.ac.uk/pdbsum/4kph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kph ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
We have previously described two types of protective B cell epitopes in the O-antigen (OAg) of the Gram negative bacterium Francisella tularensis: repeating internal epitopes targeted by the vast majority of anti-OAg monoclonal antibodies (mAbs), and a non-overlapping epitope at the nonreducing end targeted by the previously unique IgG2a mAb FB11. We now generated and characterized three mAbs specific for the nonreducing end of F. tularensis OAg, partially encoded by the same variable region germline genes, indicating that they target the same epitope. Like FB11, the new mAbs, Ab63 (IgG3), N213 (IgG3), and N62 (IgG2b), had higher antigen-binding bivalent avidity than internal-binding anti-OAg mAbs, and an oligosaccharide containing a single OAg repeat was sufficient for optimal inhibition of their antigen-binding. The X-ray crystal structure of N62 Fab showed that the antigen-binding site is lined mainly by aromatic amino acids that form a small cavity, which can accommodate no more than one and a third sugar residues, indicating that N62 binds mainly to the terminal Qui4NFm residue at the nonreducing end of OAg. In efficacy studies with mice infected intranasally with the highly virulent F. tularensis strain SchuS4, N62, N213 and Ab63 prolonged survival and reduced blood bacterial burden. These results yield insights into how antibodies to nonreducing ends of microbial polysaccharides can contribute to immune protection despite the smaller size of their target epitopes compared with antibodies to internal polysaccharide regions. This article is protected by copyright. All rights reserved.

The binding sites of monoclonal antibodies to the nonreducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide.,Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Wang Q, Costello CE, Zaia J, Seaton BA, Sharon J Immunology. 2013 Jul 11. doi: 10.1111/imm.12150. PMID:23844703<ref>PMID:23844703</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4kph" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Costello CE]]
[[Category: Lu Z]]
[[Category: Madico G]]
[[Category: Perkins HM]]
[[Category: Rynkiewicz MJ]]
[[Category: Seaton BA]]
[[Category: Sharon J]]
[[Category: Wang Q]]
[[Category: Yang C-Y]]
[[Category: Zaia J]]