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==A superfast recovering full-length LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state)==
<StructureSection load='4kuk' size='340' side='right'caption='[[4kuk]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dinoroseobacter_shibae_DFL_12_=_DSM_16493 Dinoroseobacter shibae DFL 12 = DSM 16493]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KUK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=NMM:(2S)-2-AMINO-5-[(N-METHYLCARBAMIMIDOYL)AMINO]PENTANOIC+ACID'>NMM</scene>, <scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kuk OCA], [https://pdbe.org/4kuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kuk RCSB], [https://www.ebi.ac.uk/pdbsum/4kuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kuk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8LP63_DINSH A8LP63_DINSH] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Light, oxygen, voltage (LOV) domains are widely distributed in plants, algae, fungi, bacteria, and represent the photo-responsive domains of various blue-light photoreceptor proteins. Their photocycle involves the blue-light triggered adduct formation between the C(4a) atom of a non-covalently bound flavin chromophore and the sulfur atom of a conserved cysteine in the LOV sensor domain. LOV proteins show considerable variation in the structure of N- and C-terminal elements which flank the LOV core domain, as well as in the lifetime of the adduct state. RESULTS: Here, we report the photochemical, structural and functional characterization of DsLOV, a LOV protein from the photoheterotrophic marine alpha-proteobacterium Dinoroseobacter shibae which exhibits an average adduct state lifetime of 9.6 s at 20 degrees C, and thus represents the fastest reverting bacterial LOV protein reported so far. Mutational analysis in D. shibae revealed a unique role of DsLOV in controlling the induction of photopigment synthesis in the absence of blue-light. The dark state crystal structure of DsLOV determined at 1.5 A resolution reveals a conserved core domain with an extended N-terminal cap. The dimer interface in the crystal structure forms a unique network of hydrogen bonds involving residues of the N-terminus and the beta-scaffold of the core domain. The structure of photoexcited DsLOV suggests increased flexibility in the N-cap region and a significant shift in the Calpha backbone of beta strands in the N- and C-terminal ends of the LOV core domain. CONCLUSIONS: The results presented here cover the characterization of the unusual short LOV protein DsLOV from Dinoroseobacter shibae including its regulatory function, extremely fast dark recovery and an N-terminus mediated dimer interface. Due to its unique photophysical, structural and regulatory properties, DsLOV might thus serve as an alternative model system for studying light perception by LOV proteins and physiological responses in bacteria.

Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae.,Endres S, Granzin J, Circolone F, Stadler A, Krauss U, Drepper T, Svensson V, Knieps-Grunhagen E, Wirtz A, Cousin A, Tielen P, Willbold D, Jaeger KE, Batra-Safferling R BMC Microbiol. 2015 Feb 14;15(1):30. doi: 10.1186/s12866-015-0365-0. PMID:25887755<ref>PMID:25887755</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4kuk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dinoroseobacter shibae DFL 12 = DSM 16493]]
[[Category: Large Structures]]
[[Category: Phototropin]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Batra-Safferling R]]
[[Category: Circolone F]]
[[Category: Drepper T]]
[[Category: Endres S]]
[[Category: Granzin J]]
[[Category: Jaeger K-E]]
[[Category: Knieps-Gruenhagen E]]
[[Category: Krauss U]]
[[Category: Stadler A]]
[[Category: Willbold D]]
[[Category: Wirtz A]]