View source for 4kxv


==Human transketolase in covalent complex with donor ketose D-xylulose-5-phosphate, crystal 1==
<StructureSection load='4kxv' size='340' side='right'caption='[[4kxv]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kxv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DX5:D-XYLITOL-5-PHOSPHATE'>DX5</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxv OCA], [https://pdbe.org/4kxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxv RCSB], [https://www.ebi.ac.uk/pdbsum/4kxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TKT_HUMAN TKT_HUMAN] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
It is recognized widely that enzymes promote reactions by providing a pathway that proceeds through a transition state of lower energy. In principle, further rate enhancements could be achieved if intermediates are prevented from relaxing to their lowest energy state, and thereby reduce the barrier to the subsequent transition state. Here, we report sub-angstrom-resolution crystal structures of genuine covalent reaction intermediates of transketolase. These structures reveal a pronounced out-of-plane distortion of over 20 degrees for the covalent bond that links cofactor and substrate, and a specific elongation of the scissile substrate carbon-carbon bond (d &gt; 1.6 A). To achieve these distortions, the protein's conformation appears to prevent relaxation of a substrate-cofactor intermediate. The results implicate a reduced barrier to the subsequent step that is consistent with an intermediate of raised energy and leads to a more efficient overall process.

Sub-angstrom-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate.,Ludtke S, Neumann P, Erixon KM, Leeper F, Kluger R, Ficner R, Tittmann K Nat Chem. 2013 Sep;5(9):762-7. doi: 10.1038/nchem.1728. Epub 2013 Aug 18. PMID:23965678<ref>PMID:23965678</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4kxv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transketolase 3D structures|Transketolase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ficner R]]
[[Category: Luedtke S]]
[[Category: Neumann P]]
[[Category: Tittmann K]]