View source for 4kzv


==Structure of the carbohydrate-recognition domain of the C-type lectin mincle bound to trehalose==
<StructureSection load='4kzv' size='340' side='right'caption='[[4kzv]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kzv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KZV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzv OCA], [https://pdbe.org/4kzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kzv RCSB], [https://www.ebi.ac.uk/pdbsum/4kzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E1BHM0_BOVIN E1BHM0_BOVIN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Binding of the macrophage lectin mincle to trehalose dimycolate, a key glycolipid virulence factor on the surface of Mycobacterium tuberculosis and Mycobacterium bovis, initiates responses that can lead both to toxicity and to protection of these pathogens from destruction. Crystallographic structural analysis, site-directed mutagenesis, and binding studies with glycolipid mimics have been used to define an extended binding site in the C-type carbohydrate-recognition domain (CRD) of bovine mincle that encompasses both the headgroup and a portion of the attached acyl chains. One glucose residue of the trehalose Glcalpha1-1Glcalpha headgroup is liganded to a Ca2+ in a manner common to many C-type CRDs, while the second glucose residue is accommodated in a novel secondary binding site. The additional contacts in the secondary site lead to a 36-fold higher affinity for trehalose compared to glucose. An adjacent hydrophobic groove, not seen in other C-type CRDs, provides a docking site for one of the acyl chains attached to the trehalose, which can be targeted with small molecule analogues of trehalose dimycolate that bind with 52-fold higher affinity than trehalose. The data demonstrate how mincle bridges between the surfaces of the macrophage and the mycobacterium and suggest the possibility of disrupting this interaction. In addition, the results may provide a basis for design of adjuvants that mimic the ability of mycobacteria to stimulate a response to immunization that can be employed in vaccine development.

Mechanism for recognition of an unusual mycobacterial glycolipid by the macrophage receptor mincle.,Feinberg H, Jegouzo SA, Rowntree TJ, Guan Y, Brash MA, Taylor ME, Weis WI, Drickamer K J Biol Chem. 2013 Aug 19. PMID:23960080<ref>PMID:23960080</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4kzv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Large Structures]]
[[Category: Brash MA]]
[[Category: Drickamer K]]
[[Category: Feinberg H]]
[[Category: Guan Y]]
[[Category: Jegouzo SAF]]
[[Category: Rowntree TJW]]
[[Category: Taylor ME]]
[[Category: Weis WI]]