View source for 4lmx


==Light harvesting complex PE555 from the cryptophyte Hemiselmis andersenii CCMP644==
<StructureSection load='4lmx' size='340' side='right'caption='[[4lmx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4lmx]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Hemiselmis_andersenii Hemiselmis andersenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LMX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DBV:15,16-DIHYDROBILIVERDIN'>DBV</scene>, <scene name='pdbligand=LYZ:5-HYDROXYLYSINE'>LYZ</scene>, <scene name='pdbligand=PEB:PHYCOERYTHROBILIN'>PEB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lmx OCA], [https://pdbe.org/4lmx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lmx RCSB], [https://www.ebi.ac.uk/pdbsum/4lmx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lmx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHEA2_HEMAN PHEA2_HEMAN] Light-harvesting photosynthetic tetrapyrrole chromophore-protein from the phycobiliprotein complex.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Observation of coherent oscillations in the 2D electronic spectra (2D ES) of photosynthetic proteins has led researchers to ask whether nontrivial quantum phenomena are biologically significant. Coherent oscillations have been reported for the soluble light-harvesting phycobiliprotein (PBP) antenna isolated from cryptophyte algae. To probe the link between spectral properties and protein structure, we determined crystal structures of three PBP light-harvesting complexes isolated from different species. Each PBP is a dimer of alphabeta subunits in which the structure of the alphabeta monomer is conserved. However, we discovered two dramatically distinct quaternary conformations, one of which is specific to the genus Hemiselmis. Because of steric effects emerging from the insertion of a single amino acid, the two alphabeta monomers are rotated by approximately 73 degrees to an "open" configuration in contrast to the "closed" configuration of other cryptophyte PBPs. This structural change is significant for the light-harvesting function because it disrupts the strong excitonic coupling between two central chromophores in the closed form. The 2D ES show marked cross-peak oscillations assigned to electronic and vibrational coherences in the closed-form PC645. However, such features appear to be reduced, or perhaps absent, in the open structures. Thus cryptophytes have evolved a structural switch controlled by an amino acid insertion to modulate excitonic interactions and therefore the mechanisms used for light harvesting.

Single-residue insertion switches the quaternary structure and exciton states of cryptophyte light-harvesting proteins.,Harrop SJ, Wilk KE, Dinshaw R, Collini E, Mirkovic T, Teng CY, Oblinsky DG, Green BR, Hoef-Emden K, Hiller RG, Scholes GD, Curmi PM Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2666-75. doi:, 10.1073/pnas.1402538111. Epub 2014 Jun 16. PMID:24979784<ref>PMID:24979784</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4lmx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hemiselmis andersenii]]
[[Category: Large Structures]]
[[Category: Curmi PMG]]
[[Category: Harrop SJ]]
[[Category: Wilk KE]]