View source for 4ltn


==Crystal structures of NADH:FMN oxidoreductase (EMOB) - FMN, NADH complex==
<StructureSection load='4ltn' size='340' side='right'caption='[[4ltn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ltn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/EDTA-degrading_bacterium_BNC1 EDTA-degrading bacterium BNC1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2vzj 2vzj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LTN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.996&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ltn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ltn OCA], [https://pdbe.org/4ltn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ltn RCSB], [https://www.ebi.ac.uk/pdbsum/4ltn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ltn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9F9T2_9PROT Q9F9T2_9PROT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
EDTA has become a major organic pollutant in the environment because of its extreme usage and resistance to biodegradation. Recently, two critical enzymes, EDTA monooxygenase (EmoA) and NADH:FMN oxidoreductase (EmoB), belonging to the newly established two-component flavin-diffusible monooxygenase family, were identified in the EDTA degradation pathway in Mesorhizobium sp. BNC1. EmoA is an FMNH2-dependent enzyme that requires EmoB to provide FMNH2 for the conversion of EDTA to ethylenediaminediacetate. To understand the molecular basis of this FMN-mediated reaction, the crystal structures of the apo-form, FMN.FMN complex, and FMN.NADH complex of EmoB were determined at 2.5 angstroms resolution. The structure of EmoB is a homotetramer consisting of four alpha/beta-single-domain monomers of five parallel beta-strands flanked by five alpha-helices, which is quite different from those of other known two-component flavin-diffusible monooxygenase family members, such as PheA2 and HpaC, in terms of both tertiary and quaternary structures. For the first time, the crystal structures of both the FMN.FMN and FMN.NADH complexes of an NADH:FMN oxidoreductase were determined. Two stacked isoalloxazine rings and nicotinamide/isoalloxazine rings were at a proper distance for hydride transfer. The structures indicated a ping-pong reaction mechanism, which was confirmed by activity assays. Thus, the structural data offer detailed mechanistic information for hydride transfer between NADH to an enzyme-bound FMN and between the bound FMNH2 and a diffusible FMN.

Crystal structures of NADH:FMN oxidoreductase (EmoB) at different stages of catalysis.,Nissen MS, Youn B, Knowles BD, Ballinger JW, Jun SY, Belchik SM, Xun L, Kang C J Biol Chem. 2008 Oct 17;283(42):28710-20. Epub 2008 Aug 12. PMID:18701448<ref>PMID:18701448</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ltn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: EDTA-degrading bacterium BNC1]]
[[Category: Large Structures]]
[[Category: Ballinger JW]]
[[Category: Belchik SM]]
[[Category: Jun S]]
[[Category: Kang C]]
[[Category: Knowles BD]]
[[Category: Nissen MS]]
[[Category: Xun L]]
[[Category: Youn B]]