View source for 4mmg


==crystal structure of YafQ mutant H87Q from E.coli==
<StructureSection load='4mmg' size='340' side='right'caption='[[4mmg]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mmg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MMG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MMG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mmg OCA], [https://pdbe.org/4mmg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mmg RCSB], [https://www.ebi.ac.uk/pdbsum/4mmg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mmg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YAFQ_ECOLI YAFQ_ECOLI] Toxic component of a toxin-antitoxin (TA) module. A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis. Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs. Ribosome-binding confers the sequence specificity and reading frame-dependence. When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with DinJ, the YafQ antitoxin. YafQ and DinJ together bind their own promoter, and by analogy to other TA modules probably repress its expression.<ref>PMID:17263853</ref> <ref>PMID:19307375</ref> <ref>PMID:19210620</ref> <ref>PMID:19707553</ref>   Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation.<ref>PMID:17263853</ref> <ref>PMID:19307375</ref> <ref>PMID:19210620</ref> <ref>PMID:19707553</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Dong YH]]
[[Category: Gao ZQ]]
[[Category: Liang YJ]]
[[Category: Liu QS]]