View source for 4moa


==Crystal structure of CRY4BA-R203Q TOXIN==
<StructureSection load='4moa' size='340' side='right'caption='[[4moa]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [https://pdbe.org/4moa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [https://www.ebi.ac.uk/pdbsum/4moa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The insecticidal feature of the three-domain Cry delta-endotoxins from Bacillus thuringiensis is generally attributed to their capability to form oligomeric pores, causing lysis of target larval midgut cells. However, the molecular description of their oligomerization process has not been clearly defined. Here a stable prepore of the 65-kDa trypsin-activated Cry4Ba mosquito-specific toxin was established through membrane-mimetic environments by forming an approximately 200-kDa octyl-beta-D-glucoside micelle-induced trimer. The SDS-resistant trimer caused cytolysis to Sf9 insect cells expressing Aedes-mALP (a Cry4Ba receptor) and was more effective than a toxin monomer in membrane perturbation of calcein-loaded liposomes. A three-dimensional model of toxin trimer obtained by negative-stain EM in combination with single-particle reconstruction at approximately 5 nm resolution showed a propeller-shaped structure with 3-fold symmetry. Fitting the three-dimensional reconstructed EM map with a 100-ns molecular dynamics-simulated Cry4Ba structure interacting with an octyl-beta-D-glucoside micelle showed relative positioning of individual domains in the context of the trimeric complex with a major protrusion from the pore-forming domain. Moreover, high-speed atomic force microscopy imaging at nanometer resolution and a subsecond frame rate demonstrated conformational transitions from a propeller-like to a globularly shaped trimer upon lipid membrane interactions, implying prepore-to-pore conversion. Real-time trimeric arrangement of monomers associated with L-alpha-dimyristoylphosphatidylcholine/3-[(3-cholamidopropyl)dimethylammonio]-2-h ydroxy-1-propanesulfonic acid bicelle membranes was also envisaged by successive high-speed atomic force microscopy imaging, depicting interactions among three individual subunits toward trimer formation. Together, our data provide the first pivotal insights into the structural requirement of membrane-induced conformational changes of Cry4Ba toxin monomers for the molecular assembly of a prepore trimer capable of inserting into target membranes to generate a lytic pore.

Potential Prepore Trimer Formation by the Bacillus thuringiensis Mosquito-specific Toxin: MOLECULAR INSIGHTS INTO A CRITICAL PREREQUISITE OF MEMBRANE-BOUND MONOMERS.,Sriwimol W, Aroonkesorn A, Sakdee S, Kanchanawarin C, Uchihashi T, Ando T, Angsuthanasombat C J Biol Chem. 2015 Aug 21;290(34):20793-803. doi: 10.1074/jbc.M114.627554. Epub, 2015 Jun 25. PMID:26112409<ref>PMID:26112409</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4moa" style="background-color:#fffaf0;"></div>

==See Also==
*[[Delta-endotoxin|Delta-endotoxin]]
*[[Pesticidal crystal protein|Pesticidal crystal protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus thuringiensis serovar israelensis]]
[[Category: Large Structures]]
[[Category: Angsuthanasombat C]]
[[Category: Chen C-J]]
[[Category: Thamwiriyasati N]]