View source for 4nby


==Crystal Structure of TcdA-A2 Bound to Two Molecules of A20.1 VHH==
<StructureSection load='4nby' size='340' side='right'caption='[[4nby]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nby]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_NAP07 Clostridioides difficile NAP07] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NBY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nby OCA], [https://pdbe.org/4nby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nby RCSB], [https://www.ebi.ac.uk/pdbsum/4nby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nby ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Clostridium difficile infection (CDI) is a serious and highly prevalent nosocomial disease in which the two large, Rho-glucosylating toxins TcdA and TcdB are the main virulence factors. We report for the first time crystal structures revealing how neutralizing and non-neutralizing single-domain antibodies (sdAbs) recognize the receptor-binding domains (RBDs) of TcdA and TcdB. Surprisingly, the complexes formed by two neutralizing antibodies recognizing TcdA do not show direct interference with the previously identified carbohydrate-binding sites, suggesting that neutralization of toxin activity may be mediated by mechanisms distinct from steric blockage of receptor binding. A camelid sdAb complex also reveals the molecular structure of the TcdB RBD for the first time, facilitating the crystallization of a strongly negatively charged protein fragment that has resisted previous attempts at crystallization and structure determination. Electrospray ionization mass spectrometry measurements confirm the stoichiometries of sdAbs observed in the crystal structures. These studies indicate how key epitopes in the RBDs from TcdA and TcdB are recognized by sdAbs, providing molecular insights into toxin structure and function, and providing for the first time a basis for the design of highly specific toxin-specific therapeutic and diagnostic agents.

Structural Basis for Antibody Recognition in the Receptor-Binding Domains of Toxins A and B from Clostridium difficile.,Murase T, Eugenio L, Schorr M, Hussack G, Tanha J, Kitova EN, Klassen JS, Ng KK J Biol Chem. 2013 Dec 5. PMID:24311789<ref>PMID:24311789</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4nby" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridioides difficile NAP07]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Eugenio L]]
[[Category: Hussack G]]
[[Category: Kitova E]]
[[Category: Klassen JS]]
[[Category: Murase T]]
[[Category: Ng KKS]]
[[Category: Schorr M]]
[[Category: Tanha J]]