View source for 4ndo


==Crystal structure Molybdenum Storage Protein with fully Mo-loaded cavity==
<StructureSection load='4ndo' size='340' side='right'caption='[[4ndo]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ndo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii_DJ Azotobacter vinelandii DJ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NDO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ndo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ndo OCA], [https://pdbe.org/4ndo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ndo RCSB], [https://www.ebi.ac.uk/pdbsum/4ndo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ndo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MOSB_AZOVD MOSB_AZOVD] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The molybdenum storage protein (MoSto) can store more than 100 Mo or W atoms as discrete polyoxometalate (POM) clusters. Here, we describe the three POM cluster sites along the threefold axis of the protein complex based on four X-ray structures with slightly different polyoxomolybdate compositions between 1.35 and 2A resolution. In contrast to the Moalpha-out binding site occupied by an Mo3 cluster, the Moalpha-in and Mobeta binding sites contain rather weak and non-uniform electron density for the Mo atoms (but clearly identifiable by anomalous data), suggesting the presence of POM cluster ensembles and/or degradation products of larger aggregates. The "Moalpha-in cluster ensemble" was interpreted as an antiprism-like Mo6 species superimposed with an Mo7 pyramide and the "Mobeta cluster ensemble" as an Mo13 cluster (present mostly in a degraded form) composed of a pyramidal Mo7 and a Mo3 building block linked by three spatially separated MoOx units. Inside the ball-shaped Mo13 cluster sits an occluded central atom, perhaps a metal ion. POM cluster formation at the Moalpha-in and Mobeta sites appears to be driven by filtering out and binding/protecting self-assembled transient species complementary to the protein template.

Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein.,Poppe J, Warkentin E, Demmer U, Kowalewski B, Dierks T, Schneider K, Ermler U J Inorg Biochem. 2014 Sep;138:122-8. doi: 10.1016/j.jinorgbio.2014.05.009. Epub, 2014 May 28. PMID:24945101<ref>PMID:24945101</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ndo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Azotobacter vinelandii DJ]]
[[Category: Large Structures]]
[[Category: Demmer U]]
[[Category: Ermler U]]
[[Category: Poppe J]]
[[Category: Warkentin E]]