View source for 4ndt


==Crystal structure of L. decastes alpha-galactosyl-binding lectin, orthorhombic crystal form==
<StructureSection load='4ndt' size='340' side='right'caption='[[4ndt]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ndt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lyophyllum_decastes Lyophyllum decastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NDT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.03&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ndt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ndt OCA], [https://pdbe.org/4ndt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ndt RCSB], [https://www.ebi.ac.uk/pdbsum/4ndt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ndt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AGBL_LYODE AGBL_LYODE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the alpha-galactosyl binding Lyophyllum decastes lectin (LDL) was determined to 1.0 A resolution by Sulfur-SAD (Single-wavelength Anomalous Diffraction). The 10 kDa protein exhibits no sequence similarity to any protein with known structure and adopts a unique lectin fold, where a core of two antiparallel beta-sheets at the heart of the homodimer is connected to the periphery of the structure by intramolecular disulfide bridges. This fold suggests that LDL is secreted, which sets it apart from other mushroom lectins. Structures of complexes between LDL and the ligands alpha-methylgalactoside and globotriose shed light on the binding specificity. Sequence comparison suggests a location and function of LDL and homologous proteins in or at the fungal cell wall. Structural comparison allows the identification of a superfamily of secreted proteins with the LDL fold, which may play a role at the interface between fungi and their environment.

Atomic-resolution Structure of the alpha-galactosyl binding Lyophyllum decastes lectin reveals a new protein family found in both fungi and plants.,van Eerde A, Grahn EM, Winter HC, Goldstein IJ, Krengel U Glycobiology. 2014 Dec 11. pii: cwu136. PMID:25504801<ref>PMID:25504801</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ndt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Lyophyllum decastes]]
[[Category: Grahn E]]
[[Category: Krengel U]]
[[Category: Van Eerde A]]