View source for 4nja


==Crystal structure of Fab 6C8 in complex with MPTS==
<StructureSection load='4nja' size='340' side='right'caption='[[4nja]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nja]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.204&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2M9:8-METHOXYPYRENE-1,3,6-TRISULFONIC+ACID'>2M9</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nja OCA], [https://pdbe.org/4nja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nja RCSB], [https://www.ebi.ac.uk/pdbsum/4nja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nja ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KV3A8_MOUSE KV3A8_MOUSE] [https://www.uniprot.org/uniprot/Q52L95_MOUSE Q52L95_MOUSE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
While adaptive mutations can bestow new functions on proteins via the introduction or optimization of reactive centers, or other structural changes, a role for the optimization of protein dynamics also seems likely but has been more difficult to evaluate. Antibody (Ab) affinity maturation is an example of adaptive evolution wherein the adaptive mutations may be identified and Abs may be raised to specific targets that facilitate the characterization of protein dynamics. Here, we report the characterization of three affinity matured Abs that evolved from a common germline precursor to bind the chromophoric antigen (Ag), 8-methoxypyrene-1,3,6-trisulfonate (MPTS). In addition to characterizing the sequence, molecular recognition, and structure of each Ab, we characterized the dynamics of each complex by determining their mechanical response to an applied force via three-pulse photon echo peak shift (3PEPS) spectroscopy and deconvoluting the response into elastic, anelastic, and plastic components. We find that for one Ab, affinity maturation was accomplished via the introduction of a single functional group that mediates a direct contact with MPTS and results in a complex with little anelasticity or plasticity. In the other two cases, more mutations were introduced but none directly contact MPTS, and while their effects on structure are subtle, their effects on anelasticity and plasticity are significant, with the level of plasticity correlated with specificity, suggesting that the optimization of protein dynamics may have contributed to affinity maturation. A similar optimization of structure and dynamics may contribute to the evolution of other proteins.

Adaptive Mutations Alter Antibody Structure and Dynamics during Affinity Maturation.,Adhikary R, Yu W, Oda M, Walker RC, Chen T, Stanfield RL, Wilson IA, Zimmermann J, Romesberg FE Biochemistry. 2015 Mar 24;54(11):2085-93. doi: 10.1021/bi501417q. Epub 2015 Mar, 10. PMID:25756188<ref>PMID:25756188</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4nja" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Romesberg FE]]
[[Category: Stanfield RL]]
[[Category: Wilson IA]]
[[Category: Zimmermann J]]